We narrowed to 6,522 results for: sas;

1. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMP-MBD3_1

   Plasmid

   #36371

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD3 (MBD3 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. Full human ELL2

   Plasmid

   #127253

   Purpose

   Expresses full-length human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   Other

   Tags

   His6X

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pegRNA_HEK4_20-7

   Plasmid

   #201974

   Purpose

   Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   pegRNA_HEK4_20-7
   

   Expression

   Mammalian

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSMP-MBD3_2

   Plasmid

   #36372

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MBD3 (MBD3 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSMP-G9A_2

   Plasmid

   #36332

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   G9A (EHMT2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. -4200GAMT-P1-pGL4

   Plasmid

   #26804

   Depositor

   Sam Lee

   Article

   Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

   Insert

   Guanidinoacetate N-methyltransferase (GAMT Human)
   

   Use

   Luciferase

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pMSCV tubulin myc

   Plasmid

   #12581

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   tubulin (TUBG1 Human)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   *

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMP-G9A_3

    Plasmid

    #36333

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1p shDna2

    Plasmid

    #31951

    Depositor

    Sheila Stewart

    Article

    Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

    Insert

    shRNA against hDna2 (DNA2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pegRNA_HEK4_G-T

    Plasmid

    #201975

    Purpose

    Express a pegRNA used for G to T conversion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_G-T
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pegRNA_HEK4_1bp-deletion

    Plasmid

    #201976

    Purpose

    Express a pegRNA used for 1bp deletion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMP-MBD3_3

    Plasmid

    #36373

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD3 (MBD3 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. PB-Lmo2

    Plasmid

    #60666

    Purpose

    PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Lmo2 (Lmo2 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMP-EED_2

    Plasmid

    #36386

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EED (EED Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-uTEV1-ACBD5

    Plasmid

    #171008

    Purpose

    HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-ACBD5(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMP-DNMT3A_1

    Plasmid

    #36380

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only