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pCIS.MTRIA-NE

Plasmid

#184608

Purpose

For expression of a fluorescent sensor for norepinephrine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NE

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcS-pep1-C1C2

Plasmid

#178428

Purpose

pep1 as cell penetrating peptide fused to EV membrane binging domain (C1C2)

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

pep1

Expression

Mammalian

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FRB-tTA-Myc

Plasmid

#177911

Purpose

FRB-tTA-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-tTA

Tags

Myc tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-24

Plasmid

#79939

Purpose

PH-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-24

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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TS245_pNKI-2-13_EGFP-DPN7

Plasmid

#200719

Purpose

producing Methyl Tracer EGFP protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

eGFP-DPN7

Tags

6xHis

Expression

Bacterial

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcS-p88-C1C2

Plasmid

#178424

Purpose

p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

p88

Expression

Mammalian

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
fabp10a:ER-CreER; cryaa:mCerulean

Plasmid

#230077

Purpose

Expression of ERCreER in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al NPJ Regen Med. 2026 Mar 30. doi: 10.1038

Insert

ERT2-Cre-ERT2

Use

Zebrafish expression

Promoter

fabp10a

Available Since

May 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRBBm59

Plasmid

#48115

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11

Plasmid

#79926

Purpose

Palm1-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pMEH275 NLS dTomato

Plasmid

#168210

Purpose

Mammalian expression of nuclear-localized dTomato

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-pHlash

Plasmid

#61552

Purpose

Expression of pHlash in mammalian cells

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-DA

Plasmid

#184603

Purpose

For expression of a fluorescent sensor for dopamine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-DA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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