We narrowed to 13,226 results for: GAN

1. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-pSMANTIS_SMAD-FOS_Mutant

   Plasmid

   #194188

   Purpose

   Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   mutated SMAD/FOS Site, Region 3-15nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-pSMANTIS_STAT1-2_Mutant

   Plasmid

   #194189

   Purpose

   Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   mutated STAT1/2 site, Region 653-673nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VRER-P2A-GFP-PGK-Puro

   Plasmid

   #110862

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-HF1-P2A-GFP-PGK-Puro

   Plasmid

   #110863

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-PHD2-181-426

   Plasmid

   #223551

   Purpose

   His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-180.

   Promoter

   trc

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

   Plasmid

   #194190

   Purpose

   Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   5 mutated KLF binding sites (positions 449,548,73…

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-pSMANTIS_1kb_KLF2mut 899

   Plasmid

   #194193

   Purpose

   Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   mutated KLF binding sites (position 899)

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid

    #194195

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 732)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid

    #194196

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 884)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid

    #194197

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 449)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid

    #194191

    Purpose

    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid

    #194192

    Purpose

    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    3 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid

    #194194

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 548)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-SNAP-hCB2_IRES_EGFP

    Plasmid

    #223506

    Purpose

    Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

    Depositor

    James Frank

    Article

    Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

    Insert

    SNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)
    

    Use

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid

    #169161

    Purpose

    To express nsp10-nsp14 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV6-MPP8-10xHis

    Plasmid

    #194178

    Purpose

    Expresses MPP8-10xHis tagged

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET11a_3xFlag-nsp10 (SARS-CoV-2)

    Plasmid

    #169157

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1+HIF1a-AS1

    Plasmid

    #194172

    Purpose

    Expresses lncRNA HIF1a-AS1 (spliced)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1+HIF1a-AS1_deltaExon2

    Plasmid

    #194173

    Purpose

    Expresses lncRNA HIF1a-AS1 Exon1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Exon2 was deleted

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1+HIF1a-AS1_deltaExon1

    Plasmid

    #194174

    Purpose

    Expresses lncRNA HIF1a-AS1 Exon2

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Exon1 was deleted

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

    Plasmid

    #194175

    Purpose

    Expresses lncRNA HIF1a-AS1 mutant

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within Exon1, nucleotides 26-78 were removed

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1+HIF1a-AS1_1200_unspliced

    Plasmid

    #194176

    Purpose

    Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV6-MPP8-W80A-10xHis

    Plasmid

    #194179

    Purpose

    Expresses MPP8-W80A_10xHis

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-W80A inside chromo domain

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV6-MPP8-delta55-118-10xHis

    Plasmid

    #194180

    Purpose

    Expresses MPP8-delta55-118_10xHis

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta55-118 (chromo domain deletion)

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV6-MPP8-delta2-441-10xHis

    Plasmid

    #194181

    Purpose

    Expresses MPP8-delta2-441_10xHis

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta2-441

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge