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Plasmid#205765PurposeExpression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)DepositorInsertERK KTR rsFastLime
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_hMela(CT+IL3 mGluR6)
Plasmid#191343PurposeOptogenetic toolDepositorInserthMela(CT+IL3 mGluR6)
UseTagsIRES_TurboExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_hMela(CT mGluR6)
Plasmid#191344PurposeOptogenetic toolDepositorInserthMela(CTmGluR6)
UseTagsIRES_TurboExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
Plasmid#227773PurposeExpression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafishDepositorInsertsCas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and tp1Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8270 LentiCRISPR v2 hygro sgSIGIRR-3
Plasmid#193979PurposeExpression of spCas9 and sgRNA targeting SIGIRRDepositorInsertspCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A
Plasmid#222648PurposeVector for cloning gene of interest for in vitro transcriptionDepositorInsertZsGreen
UseIn vitro transcriptionTagsExpressionMutationmutated stop codon prior to a P2A peptidePromoterT7Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: NES-FKBP-iLID-LRP6c(△1-64)
Plasmid#221775PurposeExpresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cellsDepositorInsertpCMV:: NES-FKBP-iLID-LRP6c
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_CMV_Prss-Halo-KDEL
Plasmid#216256PurposeCMV promoter-regulated ER targeted HaloTag expression vectorDepositorInsertER retained HaloTag
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-Q61L
Plasmid#214292PurposeExpress constitutively active Rac1 fused with MBPDepositorInsertConstitutively active Rac1 (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-T17N
Plasmid#214293PurposeExpress dominant negative Rac1 fused with MBPDepositorInsertDominant negative Rac1 (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-T17NPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-SMCR8
Plasmid#83440PurposeExpresses Cas9 and a gRNA targeting SMCR8DepositorInsertgRNA targeting human SMCR8 (SMCR8 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
Plasmid#221776PurposeExpresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cellsDepositorInsertpCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB
Plasmid#219557PurposePM-targeted iLID-LOVPLDDepositorInsertlyn10-iLID-LOVPLD
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTF-FLAG
Plasmid#217695PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-429PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG
Plasmid#217722PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-590PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTF-FLAG
Plasmid#217692PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4 (ADGRE4P Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-173PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG
Plasmid#217728PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF2 (ADGRF2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-435PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1-FLAG
Plasmid#217678PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA1 (ADGRA1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Key
Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_41BBICD
Plasmid#197097PurposeThis plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of 4-1BB.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_CD28ICD
Plasmid#197098PurposeThis plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of CD28.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-PL Key
Plasmid#216504PurposeRas activity dependent proximity labeler, Key only, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-PL Key
Plasmid#216505PurposeRas activity dependent proximity labeler, Key only, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-PL Key
Plasmid#216506PurposeRas activity dependent proximity labeler, Key only, ER localizedDepositorInsertER-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-PL Key
Plasmid#216507PurposeRas activity dependent proximity labeler, Key only, mitochondria localizedDepositorInsertMito-Ras-LOCKR-PL
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRAB14-HA
Plasmid#214165PurposeTransient expression of RAB14DepositorInsertRAB14 (Rab14 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Talin1-mCherry
Plasmid#212004PurposeExpression of mouse full-length (1-2541) talin1-mCherry in mammalian cellsDepositorInsertMouse talin1 (Tln1 Mouse)
UseTagsmCherry fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-P2A-Puro
Plasmid#211645PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF226_EFS-Cas9-P2A-Puro
Plasmid#211644PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (R4-R3) PRB
Plasmid#206164PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assemblyDepositorInsertProgesteron receptor B (PGR Human)
UseGateway cloning vectorTagsExpressionMutationPromoter-Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE-Per_sig_pep
Plasmid#202152PurposeLevel 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.DepositorInsertPeroxisome signal peptide
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP hSmad4-HomDonor
Plasmid#206044PurposeDonor plasmid for CRISPR knock-inDepositorInsertSmad4 (SMAD4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-TEV219S153N
Plasmid#201488PurposeTANGO detection of V5-tagged proteinDepositorInsertNbV5-TEV219S153N
UseTagsTEV219 S153NExpressionMammalianMutationS153N in TEV219PromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M4
Plasmid#203320PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hMuc18 fused to Ig like C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M2
Plasmid#203318PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hMuc18 fused to Ig like C1-C3 of hB…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M1
Plasmid#203317PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hMuc18 fused to Ig like V2-C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B4
Plasmid#203316PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hBCAM fused to Ig like C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B3
Plasmid#203315PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hBCAM fused to Ig like C2-C3 of hMUC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B1
Plasmid#203313PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hBCAM fused to Ig like V2-C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M3
Plasmid#203319PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B2
Plasmid#203314PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hBCAM fused to Ig like C1-C3 of hMU…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-Mss4D636K-Kina
Plasmid#202725Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mss4D636K-Kina
Plasmid#202740Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only