We narrowed to 2,550 results for: CCS

1. pJMP1346

   Plasmid

   #119274

   Purpose

   knockdown folA in K. pneumoniae

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA folA (K. pneumoniae)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJMP1341

   Plasmid

   #119272

   Purpose

   control sgRNA in cells lacking rfp

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOUPc-UL148-HA

   Plasmid

   #179343

   Purpose

   All-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tail

   Depositor

   Jeremy Kamil

   Article

   Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00

   Insert

   human cytomegalovirus UL148 fused to HA epitope
   

   Use

   Lentiviral and Synthetic Biology; Hiv-1 based rep…

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   insert matches UL148 from strain TB40/E

   Promoter

   Tet responsive element 3G

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJMP1219

   Plasmid

   #119259

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tet-pLKO.1_puro_shJUND #1

   Plasmid

   #136581

   Purpose

   Expresses an inducible short hairpin targeting human JUND sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shJUNDv1 (JUND Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti GW-NL-myc

   Plasmid

   #113455

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   NanoLuc-cmyc

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-YOD1-MutS2-C160S

   Plasmid

   #85668

   Purpose

   Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   YOD1 (YOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C160S and I292Q and V295Q

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-YOD1-MutS2

   Plasmid

   #85667

   Purpose

   Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   YOD1 (YOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   I292Q and V295Q

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_ACTB CRISPIE

   Plasmid

   #172853

   Purpose

   Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Inserts

   Actb intron 1 sgRNA
   DRS-2 sgRNA
   CRISPIE designer exon (phase 0-0) encoding mEGFP
   mRuby3
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP1344

    Plasmid

    #119273

    Purpose

    knockdown folA in E. aerogenes

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (E. aerogenes)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1273

    Plasmid

    #119265

    Purpose

    E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    E. coli attTn7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMP1106

    Plasmid

    #119249

    Purpose

    construction intermediate

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 myc-mβTrCP F-box (1-247)

    Plasmid

    #62976

    Purpose

    Expresses myc-tagged mouse βTrCP residues 1-247

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    3x myc

    Expression

    Mammalian

    Mutation

    codes for residues 1-247

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. GIV-FLAG S245D (SD)

    Plasmid

    #86096

    Purpose

    Expression GIV in mammalian cell

    Depositor

    Pradipta Ghosh

    Article

    Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    S245D

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti PA-mCit-NL

    Plasmid

    #113452

    Purpose

    Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMIR-Report-Luc-KLF4-K5S

    Plasmid

    #34598

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Fragment 1781-2086 of KLF4

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 PA-mCit-NL

    Plasmid

    #113444

    Purpose

    ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge