We narrowed to 157,076 results for: Des

1. pDEST-DHFR F[3]-N (LEU2)

   Plasmid

   #177798

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6P1-DEST-Strep2

   Plasmid

   #119753

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST47-ARL4C-GFP

   Plasmid

   #67402

   Purpose

   Mammalian expression of hArl4c with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL7 (ARL4C Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. 854-Desmocollin 1a-myc

   Plasmid

   #32231

   Depositor

   Kathleen Green

   Article

   Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

   Insert

   Desmocollin 1a (DSC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human beta-actin

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLenti Dest PuroR R1-R2

   Plasmid

   #84575

   Purpose

   Gateway destination vector expressing Puromycin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST-GST-hSYK

   Plasmid

   #211892

   Purpose

   Express human SYK in mammalian cells

   Depositor

   Haian Fu

   Insert

   SYK (SYK Human)
   

   Tags

   GST tag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-Flag-FBW7K404R

   Plasmid

   #200717

   Purpose

   The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   FBW7 K404 is mutated to Alanine.

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF-DEST51-GOLPH3

   Plasmid

   #21690

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Invitrogen gateway destination vector

   Tags

   6xHIS and V5

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-DEST53-mTrific

   Plasmid

   #51031

   Purpose

   mammalian expression of mouse Trific fused to GFP

   Depositor

   Timothy Cox

   Article

   Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

   Insert

   Trific (Trim46 Mouse)
   

   Tags

   Cycle3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX6P1-DEST-FLAG

    Plasmid

    #119754

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Tags

    FLAG and GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti6/V5-DEST-ANLN

    Plasmid

    #31203

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ANLN (ANLN Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

    Plasmid

    #200014

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST47-SARA-GFP

    Plasmid

    #67410

    Purpose

    Mammalian expression of hSara with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDEST47-ARF6-GFP

    Plasmid

    #67394

    Purpose

    Mammalian expression of hArf6 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Desmoplakin II-YPet(short)

    Plasmid

    #118714

    Purpose

    The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    Desmoplakin II-YPet(short) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST27-alpha-catenin

    Plasmid

    #20139

    Depositor

    Valeri Vasioukhin

    Article

    Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

    Insert

    pDEST27-alpha catenin
    

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pDN0604 (pDEST, HygR)

    Plasmid

    #210483

    Purpose

    pDEST vector with HygR marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge