Addgene

Showing: 1261 - 1280 of 9573 results

Results per page:

Sort results by:

pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMO2_Bacteroidetes-bGSDM-TEV

Plasmid

#222101

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Bacteroidetes-bGSDM-TEV ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

TEV protease site replaces residues E242–G248

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV-CX4.3-7aa-mRuby3

Plasmid

#74254

Purpose

Mammalian expression of C-terminal fusion of mRuby3 to CX43

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSYN-PC5-003

Plasmid

#42501

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

PC5 (PCSK5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV-mRuby3-18aa-Tubulin

Plasmid

#74256

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-His-Halo-Tev-PTEN

Plasmid

#58241

Purpose

expresses halo tagged PTEN protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

phosphatase and tensin homolog (PTEN Human)

Use

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_GEM4-Halo-FRB

Plasmid

#197057

Purpose

Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_GEM7-Halo-FRB

Plasmid

#197058

Purpose

Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_GEM22-Halo-FRB

Plasmid

#197059

Purpose

Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_FRB-GEM23-Halo

Plasmid

#197060

Purpose

Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

I3-01

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

actin

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-Archer1-EGFP

Plasmid

#60423

Purpose

Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

Depositor

Viviana Gradinaru

Article

Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

Insert

Archer1-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAF0690 Ef1a-Kp03_attB-BFP acceptor

Plasmid

#193473

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-Arch-GFP

Plasmid

#37810

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

Plasmid

#197067

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

MitoTrap-mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV-mClover3-18aa-Tubulin

Plasmid

#74253

Purpose

Mammalian expression of N-terminal fusion of mClover3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-cFos

Plasmid

#193089

Purpose

constitutive expression of mouse cFos in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

cFos

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge