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pLV[Exp]-Neo-EF1A>Tet3G

Plasmid

#184379

Purpose

The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Tet3G

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-mIRBP156-CHER-WPRE

Plasmid

#44351

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

RK-GFP
mIRBP156-CHER

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2554-2850) and mouse …

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP1,3 #2515

Plasmid

#74237

Purpose

Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-delStem1

Plasmid

#177616

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Mutation

3-nucleotide (UAC) deletion in Stem 1

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP2_AS #2562

Plasmid

#74238

Purpose

Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP2-AS

Use

AAV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-delSS

Plasmid

#177615

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Mutation

Slippery sequence deleted

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-DIO-tTA

Plasmid

#127091

Purpose

An AAV genome with Cre-dependent expression of tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Nix

Plasmid

#100770

Purpose

Lentiviral expression of shRNA targeting Nix

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

Lenti-sh-Nix

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pX330 LC-FKBP-Cas9

Plasmid

#162724

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)

Plasmid

#187870

Purpose

Gal4/UAS expression of dSarm(isoE)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato-f

Plasmid

#127092

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tdTomato-f

Use

AAV

Tags

farnesylation signal from c-Ha-Ras

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX330 NL-FKBP-Cas9

Plasmid

#162723

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pX330 N-FKBP-Cas9

Plasmid

#162727

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP1,3

Plasmid

#74240

Purpose

Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

Plasmid

#113684

Purpose

EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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