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Flag-SIRT1 H363Y

Plasmid

#1792

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H363Y, deacetylase domain mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28A

Plasmid

#51371

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

Plasmid

#216248

Purpose

HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human RANGAP1 (RANGAP1 Human)

Use

CRISPR and TALEN; Hdr template

Tags

miniIAA7.3xFlag.P2A.BSD

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag p21 T145A

Plasmid

#16241

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T145A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
Flag p21 T145D

Plasmid

#16242

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T145D

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-UBR5

Plasmid

#231238

Purpose

Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

UBR5 (UBR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag RbBP5

Plasmid

#15550

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pcDNAintron-CDC50A-c3xFLAG

Plasmid

#215702

Purpose

Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CDC50A (TMEM30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Sep 9. ():.

Insert

PTEN (PTEN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASx beta

Plasmid

#15210

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx beta (PIAS2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag

Plasmid

#112487

Purpose

Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3

Plasmid

#115521

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3 (Senp3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ctnnb1

Plasmid

#123586

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin3 (CUL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B Human, HDR template, Synthetic)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastbac1-Flag-FANCD2

Plasmid

#134904

Purpose

codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

FANCD2 (FANCD2 Human)

Use

Tags

Flag

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

polyhedron

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Claspin

Plasmid

#12659

Depositor

Michele Pagano

Article

Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

Insert

Claspin (CLSPN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2007

Availability

Academic Institutions and Nonprofits only