We narrowed to 5,246 results for: golden gate

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eOYW020

Plasmid

#238400

Purpose

Strongest TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P2m-U25m-TurboRFP-t0-UNSX

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCBLggPro

Plasmid

#199736

Purpose

Golden Gate entry vector for promoter cloning

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLggTerm

Plasmid

#199738

Purpose

Golden Gate entry vector for terminator cloning

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Mutation

L17E/D481V

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Mutation

WT

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLY134

Plasmid

#130962

Purpose

Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ131A2.0

Plasmid

#99884

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HS_NAM-B1

Plasmid

#154064

Purpose

Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Inserts

Hygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1

Use

Cre/Lox and Synthetic Biology; Golden gate

Expression

Bacterial and Plant

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

HvHSP17, OsAct, and ZmUbi

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1313_pPV3-WPRE

Plasmid

#239703

Purpose

BsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequence

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

WPRE 3' UTR

Use

Synthetic Biology

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pPiggyBacATCC LacZ GCTT loxAttP

Plasmid

#173496

Purpose

piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

Depositor

Eric Marois

Publication

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Type

Empty backbone

Expression

Insect

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0245

Plasmid

#162284

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Publication

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEM111

Plasmid

#248760

Purpose

TetR part vector for Golden Gate Assembly

Depositor

Carolina Tropini

Publication

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP2E5-RBS1PLP-TetR-rrnBT1

Expression

Bacterial

Available since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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