We narrowed to 5,817 results for: pCas

1. pCAG-nDsRedFL-miR124

   Plasmid

   #177806

   Purpose

   The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-124
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-GCaMP2-actin

   Plasmid

   #18928

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   GCaMP2-Actin (ACTB Chicken)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

3. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AviMta2-3xFiBsd

   Plasmid

   #140964

   Purpose

   full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis-associated gene family, member 2 (Mta2 Mouse)
   

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaSpeR-act5cB-GAL4QF

   Plasmid

   #61309

   Purpose

   Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   GAL4_DBD::QF2w_AD
   

   Expression

   Insect

   Mutation

   Changed EK on C-terminus of QF2 to KKKK

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. G1397 DddAtox-C–dSpCas9

   Plasmid

   #157836

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9–mKate2ps–CgRNA

   Plasmid

   #64955

   Purpose

   Control gRNA (GTCAAGGCACTCTTGCCTA)

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Expression

   Mammalian

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-NLGN4 D396*

   Plasmid

   #59321

   Purpose

   Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN4 D396* (NLGN4X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D396* nonsense mutation, rest of ORF deleted

   Promoter

   CAG

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Dcst2-3xHA

    Plasmid

    #183543

    Purpose

    Expression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

    Insert

    DC-STAMP domain containing 2 (Dcst2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-RFP-int

    Plasmid

    #19822

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMUT2-pCaf-mCherry

    Plasmid

    #192860

    Purpose

    pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG eGFP-Oct4

    Plasmid

    #203789

    Purpose

    Competitive assay of Oct4 and Oct6 binding to DNA motifs.

    Depositor

    Thorsten Wohland

    Article

    Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-eArch3.0-mRuby2-ST

    Plasmid

    #109135

    Purpose

    Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eArch3.0-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-S-B.1.1.529-FLAG

    Plasmid

    #180774

    Purpose

    B.1.1.529 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.1.529
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 23, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCAG1.1-Myc/TSSK1

    Plasmid

    #199624

    Purpose

    Expression vector of mouse TSSK1. Myc-tag at N-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Tssk1 mRNA (Tssk1 Mouse)
    

    Tags

    Myc-tag

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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