We narrowed to 6,263 results for: wus

1. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p23-mNeongreen-DDX21 WT-mRuby3

   Plasmid

   #179225

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO-Bcl2-IRES-tdtomato(TET005)

   Plasmid

   #117857

   Purpose

   This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

   Depositor

   C. Ron Yu

   Article

   Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

   Insert

   Bcl2 (Bcl2 Mouse)
   

   Use

   Tta/teto

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mPA-GFP-PA-Rac1

   Plasmid

   #22022

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His and mPA-GFP

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pTarget: Ptaq-RFP_PlacIq-GFP

   Plasmid

   #192280

   Purpose

   RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Inserts

   RFP
   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacIq and Ptaq

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p23-mRuby3-DDX21 WT

   Plasmid

   #179231

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTriEx-PA-Rac1-C450A

   Plasmid

   #22025

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains light-insensitive mutation C4…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-PEmax-P2A-hP53DD

   Plasmid

   #214084

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax-P2A-hP53DD (TP53 Human, Synthetic, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-PA-Rac1-I539E

   Plasmid

   #22026

   Purpose

   Expression of photoactivatable Rac1 ('lit' state mutant)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains 'lit state' mutatio…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. p23-mNeongreen-DDX21 DAT-mRuby3

    Plasmid

    #179226

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Mutation

    G234D, K235A

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTarget-RFP-GFP operon

    Plasmid

    #192279

    Purpose

    RFP GFP fusion protein expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacIq

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

    Plasmid

    #117856

    Purpose

    This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid

    #22021

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains the light-insensitive C450A m…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

    Plasmid

    #22034

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450M.Rac1 s…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. p23-mNeongreen-DDX21 HGV-mRuby3

    Plasmid

    #179227

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

    Plasmid

    #22023

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450A.Rac1 s…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pSIN-CD4

    Plasmid

    #175942

    Purpose

    knockout mouse CD4

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    CD4 gRNA (Cd4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE-cadherin-GFP-N-WASP deltaVCA

    Plasmid

    #101283

    Purpose

    Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    delta VCA domain

    Promoter

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Use

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Promoter

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p23-mNeongreen-DDX21 HGV

    Plasmid

    #179230

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge