162,717 results

1. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

   Plasmid

   #205319

   Purpose

   All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISTfu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-cCFP

   Plasmid

   #185361

   Purpose

   For mammalian expression of C-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM4Z-EGFP

   Plasmid

   #183475

   Purpose

   This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAP-delta10-cHIS

   Plasmid

   #21716

   Depositor

   Andrew Bohm

   Article

   Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

   Insert

   polyA polymerase (PAP1 Budding Yeast)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   30 amino acids off the c terminus

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-N1 human cofilin S3E

   Plasmid

   #50861

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHis17_ParM _2

   Plasmid

   #78202

   Purpose

   Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

   Insert

   parM (K33A D63C T174A T175N D224C C287A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A D63C T174A T175N D224C C287A

   Promoter

   unknown

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. P2RY1-Tango

   Plasmid

   #66468

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY1 (P2RY1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-GFAP-hM3D(Gq)-mCherry

   Plasmid

   #50478

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-LC3 (human)

    Plasmid

    #24920

    Purpose

    Mammalian expression of LC3 fused to EGFP

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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