Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

162,692 results

Showing: 1281 - 1300 of 162692 results

  1. pCDF-Frb-v1

    Plasmid
    #201923
    Purpose
    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (modified), T7 (modified, moderate), T7 (modif…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201923-plasmid-map-sequence-id-395412

    Enlarge

  8. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

    Plasmid
    #191737
    Purpose
    AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191737-plasmid-map-sequence-id-376686

    Enlarge

  11. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

    Enlarge

  13. hGli2 flag3x

    Plasmid
    #84920
    Purpose
    mammalian expression of Gli2
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli2 (GLI2 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84920-plasmid-map-sequence-id-203620

    Enlarge

  14. pYHY2

    Plasmid
    #216108
    Purpose
    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT0294 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    BfP1E6 promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216108-plasmid-map-sequence-id-434369

    Enlarge

  15. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

    Enlarge

  19. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

    Enlarge

Showing: 1281 - 1300 of 162692 results