165,775 results

1. pDONR223-NEK7

   Plasmid

   #23395

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Tobias Meyer, Sabrina Spencer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSYN-mito-dsRED2

   Plasmid

   #173069

   Purpose

   mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   human synapsin 1

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. circRNA-synIRES-R25-NanoLuc

   Plasmid

   #188116

   Purpose

   Template plasmid to transcribe circular RNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. [#GS16-LV] Syn1-MitoTRACER - Donor

   Plasmid

   #233565

   Purpose

   Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   MitoTRACER-Donor
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   GFP11 and HA Tag

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xHA-TurboID-NLS_pCDNA3

   Plasmid

   #107171

   Purpose

   expresses 3xHA-tagged TurboID in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-SFPQ

    Plasmid

    #166960

    Purpose

    Expresses FLAG-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShuttle-CMV

    Plasmid

    #16403

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-G-Flamp2

    Plasmid

    #192782

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-GenEPi

    Plasmid

    #140236

    Purpose

    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

    Depositor

    Periklis Pantazis

    Article

    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

    Insert

    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    

    Tags

    GCaMP 6s RS1 EF4

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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