We narrowed to 2,124 results for: PORA
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Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL
Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1mCNPRS
Plasmid#174718PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNEU-KlacRS
Plasmid#232155PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-TAG-EGFP
Plasmid#232156PurposeA fluorescence reporter to show incorporation of target unnatural amino acids.DepositorInsertmCherry-linker(TAG)-EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MmKlacRS1
Plasmid#232157PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-chKlacRS-WT
Plasmid#232159PurposeA chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.DepositorInsertchKlacRS-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-chKlacRS-IPYE
Plasmid#232160PurposeA chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.DepositorInsertchKlacRS-IPYE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK2(252-551)-NanoLuc Fusion Vector
Plasmid#237172PurposeExpress EIF2AK2(252-551)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsNanoLuc (R)ExpressionMammalianMutationTruncation of full-length protein; expressing res…PromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1
Plasmid#237139PurposeExpress MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS 4B(G12V) BiBiT-Ready Vector
Plasmid#237076PurposeExpress LgBiT-KRAS 4B(G12V) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12VPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS 4B G12C BiBiT Ready Vector
Plasmid#237078PurposeExpress LgBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12CPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS(G12D) Vector
Plasmid#237033PurposeExpress LgBiT-KRAS(G12D) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HiBiT-KRAS(G12C) Fusion Vector
Plasmid#236889PurposeExpress HiBiT-KRAS(G12C) in Mammalian Cells under a CMV promoterDepositorUseTagsHiBitExpressionMammalianMutationG12CPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, HaloTag-KRAS
Plasmid#236871PurposeExpress HaloTag(R)-KRAS in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)
Plasmid#236863PurposeExpress NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoterDepositorUseTagsNanoLuc (R)ExpressionMammalianMutationQ61RPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)
Plasmid#236854PurposeExpress NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R) and NanoLuc (R)ExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, HaloTag-KRAS(G13D)
Plasmid#236856PurposeExpress HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationG13DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SmBiT-KRAS4B(G12D) BiBiT-Ready Vector
Plasmid#236798PurposeExpress SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsSmBiTExpressionMammalianMutationG12DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRCA595 - pBA904 Puro-T2A-mCherry
Plasmid#238171PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsmCherryExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA594 - pBA904 Puro-T2A-BFP
Plasmid#238170PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only