We narrowed to 2,365 results for: DAP

1. pcDNA3-JNKsubDD-YPet-NES

   Plasmid

   #84633

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsubDD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_+1D

   Plasmid

   #149389

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   1bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-M2-TLR2-V660N

   Plasmid

   #12292

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Use

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I, H202Y and V660N; signal peptide (aa1-23) re…

   Promoter

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA CrkII Y221F

   Plasmid

   #50732

   Purpose

   mammalian expression of CrkII Y221F mutant

   Depositor

   Kenneth Yamada

   Article

   Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

   Insert

   CrkII Y221F (CRK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y221F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMKO.1-shPrc1

   Plasmid

   #160959

   Purpose

   Prc1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Prc1 shRNA (Prc1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-M2-TLR2-P681H

   Plasmid

   #12290

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Use

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   A74V, M82I, H202Y and P681H; signal peptide (aa1-…

   Promoter

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ets2DBM

   Plasmid

   #28129

   Depositor

   Pumin Zhang

   Article

   Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

   Insert

   Ets2 (ETS2 Human)
   

   Use

   Mouse Targeting and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The putative destruction box (RGTL) residues 20–2…

   Promoter

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-ERKsubDD-Venus-NES

    Plasmid

    #84630

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ERKsubDD-Venus-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK-flag-USP19 494-1290

    Plasmid

    #78580

    Purpose

    Expression flag-USP19 494-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 494-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-493 and mutated 1291 residue…

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. M34 Xdsh PDZ bait-pGBT9

    Plasmid

    #17190

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Xdsh (dvl2.L Frog)
    

    Use

    Tags

    Gal4-DBD

    Expression

    Yeast

    Mutation

    Dsh bp 343-1191

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. XE160 XDshHA-pt mutant PDZ-CS2+

    Plasmid

    #16780

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Dishevelled (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    HA

    Expression

    Mutation

    point mutation in the binding pocket of the PDZ b…

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. XE161 XDshHA-triple pt mutant-CS2+

    Plasmid

    #16779

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    Dishevelled (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    HA

    Expression

    Mutation

    triple point mutation outside of the PDZ binding …

    Promoter

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. EC.SurA.pTYB1.a21-281&390-428.L239R

    Plasmid

    #15959

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Escherichia coli surA delta PPIase domain 2
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    residues 282-389 deleted; L239R mutation

    Promoter

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGEMHE-RnCENP-W

    Plasmid

    #238064

    Purpose

    Expresses rat CENP-W; untagged; made for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Rat CENP-W (Cenpw Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHInt-AppA-KanR-loxP

    Plasmid

    #232910

    Purpose

    Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    AppA (appA )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pj5

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACEBac1_Strep-Psc-BICD2(711-800)_L786A

    Plasmid

    #228838

    Purpose

    Insect cell expression of the mutant C-terminus of BICD2

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800, with L786A

    Promoter

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACEBac1_Strep-Psc-BICD2(711-800)

    Plasmid

    #228833

    Purpose

    Insect cell expression of CC3 domain of BICD2 (aa 711-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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