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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMef2c-AHF-DEST (JDW 476)

Plasmid

#242577

Purpose

Gateway destination vector with the murine Mef2c anterior heart field promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBeta-MHC-promoter DV (JDW 26)

Plasmid

#229835

Purpose

A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

min-ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT5-d2EGFP-mCherry

Plasmid

#201454

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_d2EGFP

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBA407-Neo-COVGT5-mCherry

Plasmid

#201455

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_mCherry

Use

Lentiviral and Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGEN-WPRE-bGH-DEST (JDW 1309)

Plasmid

#229815

Purpose

A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP-Nt(47-173)

Plasmid

#165091

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

Plasmid

#117384

Purpose

An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Expression

Bacterial

Available Since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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