We narrowed to 24,460 results for: Spr

1. pWJ68

   Plasmid

   #46571

   Purpose

   Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   w-dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1394

   Plasmid

   #129522

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFLAG-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZ8-P_dCas9

   Plasmid

   #74063

   Purpose

   pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Propionate inducible promoter (prp)

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGB411

   Plasmid

   #134760

   Purpose

   pCambia CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYZ673

   Plasmid

   #132953

   Purpose

   crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   FnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   adh1

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL1166

   Plasmid

   #129520

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFLAG-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ221

   Plasmid

   #132952

   Purpose

   crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   FnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   adh1

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pG3GB411

   Plasmid

   #134748

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEL678

    Plasmid

    #207511

    Purpose

    GmUbi3-SpRY-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    GmUBI3

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEL679

    Plasmid

    #207512

    Purpose

    GmM4-SpRY-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    GmM4

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEL681

    Plasmid

    #207514

    Purpose

    GmM4-SpRY ABE-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    GmM4

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1211

    Plasmid

    #129521

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFlag-NLS

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIMcpf1

    Plasmid

    #153034

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasKP-hph

    Plasmid

    #117232

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Promoter

    AtRPS5A

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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