We narrowed to 26,837 results for: tat

1. pDONR223 PAK5 T538N

   Plasmid

   #60537

   Purpose

   Donor vector for human Pak5 T538N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   T538N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDONR223 PAK5 S573N

   Plasmid

   #60538

   Purpose

   Donor vector for human Pak5 S573N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   S573N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

   Plasmid

   #207025

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A I375A D384A Y387A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCUP1-GFP-Pho8-406

   Plasmid

   #207006

   Purpose

   Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PCUP1-GFP-Osh1-406

   Plasmid

   #207007

   Purpose

   Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   OSH1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

   Plasmid

   #207050

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   P85L P86L

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

   Plasmid

   #207051

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   T56A R65A T87A

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

   Plasmid

   #207052

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   R65A P85A P86A

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

   Plasmid

   #207053

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   R65A P85L P86L

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 186_pETcon_SARS2_XBB1-5

    Plasmid

    #208086

    Purpose

    yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

    Insert

    SARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR2_+10D+7U

    Plasmid

    #149393

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    7bp inserted upstream, and 10bp inserted downstre…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-Flag-stm2585_Y167F

    Plasmid

    #174398

    Purpose

    Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA
    

    Use

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Mutation

    Y167F

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Flag-stm2585_Y167F

    Plasmid

    #174392

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    stm2585
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Tyr 167 changed to Phe

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_mutNSDHL

    Plasmid

    #136428

    Purpose

    Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.

    Depositor

    Antonio Amelio

    Article

    Getz et al Oral Surg Oral Med Oral Pathol Oral Radi

    Insert

    human NSDHL with mutations in exon 4 and exon 6 (NSDHL Synthetic, Human)
    

    Use

    Tags

    V5-His tag

    Expression

    Mammalian

    Mutation

    C>T = A105V; G>C = A182P; G>A = R199H; G…

    Promoter

    CMV

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-Q842gp160-754*

    Plasmid

    #123231

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-191084-gp160-754*

    Plasmid

    #123236

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-Q769gp160-754*

    Plasmid

    #123227

    Purpose

    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-ZM197M-gp160-754*

    Plasmid

    #123220

    Purpose

    Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (ZM197M) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge