We narrowed to 17,814 results for: por
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pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast
Plasmid#205548PurposeExpress EPN11-MCP-p6-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP-p6
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
TagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108376PurposeFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-mp62
Plasmid#58785PurposeExpresses SECFP-mp62 in mammalian cellsDepositorAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII-Cas9n-UGI
Plasmid#119141PurposeBase editor made from APOBEC3H Haplotype IIDepositorInsertAPOBEC3H Haplotype II-Cas9 nickase-UGI
UseCRISPRMutationNonePromoterCMVAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
ExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only
