We narrowed to 9,389 results for: Coli

1. pCMV Lex VP16 HA (P#1708)

   Plasmid

   #14593

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Use

   Tags

   HA and VP16

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pEIAV-SIN6.1 CZW

   Plasmid

   #44172

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   LacZ (lacZ E. coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST50Trc1-DHFRHIS

   Plasmid

   #63949

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFF706

   Plasmid

   #61448

   Purpose

   Light-inducible SCRIBE_kanR(ON)

   Depositor

   Timothy Lu

   Article

   Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

   Insert

   SCRIBE_kanR(ON)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pDawn

   Available since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF/myc/ER FLIPglu-600uDelta13V

   Plasmid

   #18020

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu F16A Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   F16A in binding protein

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS513

   Plasmid

   #17283

   Depositor

   Nancy Craig

   Article

   Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

   Insert

   TnsB (tnsB E. coli transposon Tn7)
   

   Use

   Tags

   Chitin-binding domain-intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pFLIPmal-40uDelta1YFP

   Plasmid

   #18853

   Depositor

   Wolf Frommer

   Article

   Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

   Insert

   optimized maltose FRET sensor
   

   Use

   Tags

   CFP and YFP

   Expression

   Bacterial

   Mutation

   N227A;

   Promoter

   Available since

   Oct. 23, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pDR-GW FLIPglu-30μΔ13V

   Plasmid

   #27998

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-30μΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   D154A in mglB

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pECT5

   Plasmid

   #75451

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046865

    Plasmid

    #153930

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

    Plasmid

    #18016

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V Venus-Y67G
    

    Use

    Tags

    ECFP and Venus-Y67G

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional Venus

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCVD433

    Plasmid

    #63756

    Purpose

    Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs

    Depositor

    James Kaper

    Article

    Donnenberg et al Infect Immun. 1990 Jun;58(6):1565-71.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH849-gst-optec

    Plasmid

    #89590

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST50Trc4-STRHISNDHFR

    Plasmid

    #64000

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSG 12-6

    Plasmid

    #63870

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    UAG near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDUET-aaCPRct-A13AMO

    Plasmid

    #20116

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pDR-GW FLIPglu-2µΔ13V

    Plasmid

    #28000

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLIPglu-2µΔ13V
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    H152A

    Promoter

    Available since

    June 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSG 12DP

    Plasmid

    #63876

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    -1 frameshift near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pST50Trc2-STRDHFR

    Plasmid

    #63955

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Strep II (STR)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSG 34-11

    Plasmid

    #63869

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    UGA near N-term

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pFF758

    Plasmid

    #61454

    Purpose

    IPTG-inducible msd(wt)_dRT

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd(wt)_dRT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D197A D198A

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSG lacZ

    Plasmid

    #63874

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    +1 frameshift near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pECM1

    Plasmid

    #75445

    Purpose

    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTU1

    Plasmid

    #72934

    Purpose

    Backbone vector with internal BsaI removed by site-directed mutagenesis

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTKDP-hph

    Plasmid

    #71322

    Purpose

    Donor plasmid - hygromycin resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pKP660

    Plasmid

    #160812

    Purpose

    Expression of ExbB, ExbD proteins

    Depositor

    Kathleen Postle

    Article

    Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

    Insert

    exbB, exbD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pST50Trc4-STRNDHFR

    Plasmid

    #63996

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKS044

    Plasmid

    #65472

    Purpose

    pTrc plasmid expressing clpP

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    clpP (clpP )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSG 3/4

    Plasmid

    #63868

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    UGA near N-term

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pT7_circRAJ31

    Plasmid

    #69931

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    pT7_circRAJ31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pST50Trc1-HISDHFR

    Plasmid

    #63935

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BAG (CC#23)

    Plasmid

    #15148

    Depositor

    Connie Cepko

    Article

    Price et al Proc Natl Acad Sci U S A. 1987 Jan . 84(

    Insert

    beta-galactosidase
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

35. B7N6P4

    Plasmid

    #163180

    Purpose

    Inducible expression of UniProt identifier B7N6P4

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    B7N6P4
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7_circRAJ31_C873U

    Plasmid

    #69932

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    pT7_circRAJ31_C873U
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pST50Trc3-STRNDHFR

    Plasmid

    #63978

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCA-ZtTA

    Plasmid

    #26083

    Depositor

    Liqun Luo

    Article

    Li et al PLoS One. 2010 . 5(7):e11503.

    Insert

    a loxP-flanked β-geo preceding the tetracycline transactivator tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pML2003

    Plasmid

    #193255

    Purpose

    Propionate sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    prpR
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pprp and Synthetic promoter P6

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBP

    Plasmid

    #72947

    Purpose

    Backbone vector with internal BsmBI removed by site-directed mutagenesis

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge