We narrowed to 14,145 results for: TIM
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Plasmid#174325PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#2
Plasmid#174324PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#1
Plasmid#174323PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)
Plasmid#174139PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRQR=D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E04: attB4-CMV51p>-attB5
Plasmid#162973PurposeGateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized human CMV promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E36: attB4-CMV51p>-attB1r
Plasmid#162927PurposeGateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoterDepositorInsertoptimized CMV promoter
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFapO_94
Plasmid#149597PurposepXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-FapO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXylO_94
Plasmid#149598PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-XylO_94Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetO_105
Plasmid#149599PurposepXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterTEF1-TetO_105Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA271
Plasmid#149600Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA272
Plasmid#149601Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA197
Plasmid#149602Purpose(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFPDepositorInsertyeGFP
UseSynthetic BiologyExpressionYeastPromoterPcaO_1O8Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_79
Plasmid#149603Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetRDepositorInsertTetR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-2
Plasmid#136386PurposeExpresses DANCER-2 dynamic proteinDepositorInsertDANCER-2
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39LPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-S
Plasmid#136388PurposeExpresses NERD-S static proteinDepositorInsertNERD-S
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39I-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-C
Plasmid#136389PurposeExpresses NERD-C static proteinDepositorInsertNERD-C
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-F30L-V39IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM45
Plasmid#154152PurposeFirefly luciferase with no promoter for negative control for luciferase assaysDepositorInsertFirefly luciferase
UseLuciferaseAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-10
Plasmid#140139PurposeEvaluating the impact of feedback and high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
ExpressionBacterialAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_Cdkn1b.a
Plasmid#107523PurposeNegative regulator of cell cycle progression by G1/S transition inhibitionDepositorInsertCdkn1b.a
PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_Cdkn1b.b
Plasmid#107524PurposeNegative regulator of cell cycle progression by G1/S transition inhibitionDepositorInsertCdkn1b.b
PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGuide-P1-O1a (EL702)
Plasmid#140040PurposeCRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.DepositorInsertbarcode P1 and sgRNA lacO1 array
UseSynthetic BiologyAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCG 306
Plasmid#131167PurposePCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneTagsCCGExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH70
Plasmid#128190PurposeLevel1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
IgH intron enhancer
Plasmid#127247PurposeTo maximize gene expression in B cellsDepositorInsertheavy chain enhancer
ExpressionMammalianAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2963_eSpCas9-PGKHygdtkCh
Plasmid#124205PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2
Plasmid#124169PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1123_pPB-TREtight-cHA-IRESH2BmKO2
Plasmid#124172PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KFP14
Plasmid#119796PurposeRpoD6 epitope tagged construct for integration at neutral site 1DepositorInsertSynpcc7942_1557 (Synpcc7942_1557 )
ExpressionBacterialAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
KFP13
Plasmid#119795PurposeRpoD5 epitope tagged construct for integration at neutral site 1DepositorInsertSynpcc7942_1849 (Synpcc7942_1849 )
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
KFP06
Plasmid#119799PurposeRpoD6 epitope tagged construct for integration at neutral site 2.2DepositorInsertSynpcc7942_1557 (Synpcc7942_1557 )
ExpressionBacterialAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KFP05
Plasmid#119800PurposeRpoD5 epitope tagged construct for integration at neutral site 2.2DepositorInsertSynpcc7942_1849 (Synpcc7942_1849 )
ExpressionBacterialAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES945
Plasmid#112643Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertCRY2PHR-GAL4DBD
ExpressionYeastPromoterTDH3Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ReNL-Actin_pcDNA3
Plasmid#89531PurposeRed color variant of bright luminescent protein, fused to actinDepositorInsertReNL
TagsActinExpressionMammalianPromoterCMVAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVHed07
Plasmid#82350PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa
Plasmid#78183PurposeExpresses pcDronpa in E. coli strainsDepositorInsertpcDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB762
Plasmid#60150PurposeFirefly luciferase (Promega) driven by LEU1 promoter.DepositorInsertFLuc
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB767
Plasmid#60159PurposeFirefly luciferase (Promega) driven by SIC1 promoter.DepositorInsertFLuc
ExpressionYeastMutationA4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB649
Plasmid#60136PurposeFirefly luciferase (Promega) driven by MET17 promoter.DepositorInsertFLuc
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only