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pJD112
Plasmid#23088DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone 3 Changed Serine 10 to Aspartate, H3 S10D.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD113
Plasmid#23089DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Serine 10 to Glutamamte, H3 S1…PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(A509D)
Plasmid#1147DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationA509DPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(G217S)
Plasmid#1149DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationG217SPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
RMC sup35 promotor integr
Plasmid#1175DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJW1668
Plasmid#112042PurposeCEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URADepositorInsertFET3
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1669
Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.1
Plasmid#102547Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
UseTagsExpressionBacterialMutationNonePromoterT7Available sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPM47 (UPR-RFP CEN/ARS URA3)
Plasmid#20132DepositorInsertUPR-RFP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLS.436
Plasmid#184953PurposeExpress -Eco1 RT and ncRNADepositorInsertEco1: ncRNA(wt) and RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAC212
Plasmid#187912Purposepsac212-crispey-bar-ade2KODepositorInsertade2 KO CRISPEY cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorInserthH1 (hH1 Neurospora crassa)
UseTagsV5, ccg-1 promoter, and mEos3.1ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneUseTagsCRUExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPRi-D2
Plasmid#182927Purposeinducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803DepositorInsertddcpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p426*-LbSgH
Plasmid#136992PurposeHelper plasmid for LbCpf1 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMG13
Plasmid#122932PurposeExpression of the regulator tTA.DepositorInserttTA
UseTagsExpressionYeastMutationPromoterPcln3Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK59-ATG18-GFP
Plasmid#42529DepositorInsertAtg18 (ATG18 Budding Yeast)
UseTagsGFPExpressionYeastMutationnonePromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pTS1
Plasmid#87797PurposeFor constructing SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTS2
Plasmid#87798PurposeFor constructing SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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CFP-CitA6-130-Venus
Plasmid#128016PurposeUnmodified FLIP CitA6-130 sensorDepositorInsertCitA
UseTagsExpressionMutationsee Depositor CommentsPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F53
Plasmid#23077DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9RPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F30
Plasmid#23066DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 9 to Glutamine H3 K9QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F31
Plasmid#23067DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 14 to Glutamine K14QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJLI-sup35
Plasmid#1210DepositorInsertsup35 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationsup35 gene is not included in this plasmid. Only…PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSL6 - Brr2 del247 WT
Plasmid#90256Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 247 amino acidsPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDC12(K351N)
Plasmid#1163DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTags266bp of upstream sequenceExpressionYeastMutationK351NPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCuGFP-SpAtg17(416)
Plasmid#122080PurposeExpressing S. pombe GFP-Atg17DepositorInsertSpATG17 (atg17 Fission Yeast)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSARSF53-259
Plasmid#61757PurposeNpuDnaB(delta283)dC39 inteinDepositorInsertNpuDnaB(delta283)dC39 intein
UseTagsH6-Smt3ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAL-CDC12(K351N)
Plasmid#1166DepositorInsertCDC12 (CDC12 Budding Yeast)
UseTagsExpressionYeastMutationK351NPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only
