We narrowed to 6,480 results for: poly
-
Plasmid#67901PurposeExpresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cellsDepositorInsertmCherry-FKBP12-DH
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-Flag M33
Plasmid#1992DepositorInsertM33 (Cbx2 Mouse)
UseTagsFlagExpressionInsectMutationKozak sequence added before ATGPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Sfxn3
Plasmid#48169Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of SFXN3 (SFXN3 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET:CCCT:GFP
Plasmid#60738PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJKW1847
Plasmid#174004PurposeExpresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-DJ1-M26I
Plasmid#29353DepositorInsertDJ1 (PARK7 Human)
UseTagsGSTExpressionBacterialMutationMet26IlePromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD14
Plasmid#112822PurposepSMF2 with 14 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT
Plasmid#24606DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, Y459APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac K-M33-Flag
Plasmid#1993DepositorInsertM33 (Cbx2 Mouse)
UseTagsFlagExpressionInsectMutationKozak sequence added before ATGPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 short UTR
Plasmid#21644DepositorInsertCyclin D2 short UTR (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) C716A
Plasmid#24607DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, Y459A, C716APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH-HT_ygiF-FL
Plasmid#127795PurposeExpresses full-length ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8-DJ1-M26I
Plasmid#29357DepositorInsertDJ1 (PARK7 Human)
UseEntry vectorTagsExpressionMutationMet26IlePromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-DJ1-E64D
Plasmid#29352DepositorInsertDJ1 (PARK7 Human)
UseTagsGSTExpressionBacterialMutationGlu64AspPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Myc-CICf_L40S
Plasmid#48187PurposeExpresses L40S mutated CicDepositorInsertCapicua (Cic Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV Cyclin D2-long-mut
Plasmid#21657DepositorInsertCyclin D2 (CCND2 Human)
UseRetroviralTagsExpressionMammalianMutationmutated miR-15/16 and let-7 binding sitesPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
LEPG_shSik3.3974
Plasmid#125855PurposeRetroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance geneDepositorInsertshRNA targeting Sik3
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LEPG_shSik3.308
Plasmid#125878PurposeRetroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance geneDepositorInsertshRNA targeting Sik3
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-1459-adcy4-Luc
Plasmid#28184DepositorInsertAdcy4 promoter (Adcy4 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-GFP-iRFP670
Plasmid#111814PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
GFP
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY091
Plasmid#110068PurposeBacterial expression of L4DepositorInsertL4
UseTagsExpressionMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY075
Plasmid#110067PurposeBacterial expression of L2DepositorInsertL2
UseTagsExpressionBacterialMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD10
Plasmid#112821PurposepSMF2 with 10 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mut
Plasmid#48164Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Mut
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Myc-CICf_W37A
Plasmid#48186PurposeExpresses W37A mutated CicDepositorInsertCapicua (Cic Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
myc-SpEAF pBacPAK8
Plasmid#15320DepositorInsertELL associated factor (eaf1 Fission Yeast)
UseBaculovirus constructionTagsMycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR8-DJ1-D149A
Plasmid#29355DepositorInsertDJ1 (PARK7 Human)
UseEntry vectorTagsExpressionMutationAsp149AlaPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
2842 pBS Bcl-2 5'genomic
Plasmid#8747DepositorInsertBcl-2 5' genomic (Bcl2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N+C-term K-R
Plasmid#233093PurposeExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with N+C-term lysines (K) mutated to arginine (R)
UseTagsGSTExpressionBacterialMutationGST-YihI with N+C-term lysines (K) mutated to arg…PromoterAvailable sinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-All K-R
Plasmid#233094PurposeExpression of GST-YihI with all lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with all lysines (K) mutated to arginine (R)
UseTagsGSTExpressionBacterialMutationGST-YihI with all lysines (K) mutated to arginine…PromoterAvailable sinceMay 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)
Plasmid#233868PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh2
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCARGO-RMCE
Plasmid#233264PurposeBackbone plasmid used to clone lncRNA inserts in preparation for RMCEDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)
Plasmid#225328PurposeExpresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertHcdh partial gene cluster
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)
Plasmid#225314PurposeExpresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213DepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)
Plasmid#225306PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)
Plasmid#225307PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)
Plasmid#233873PurposeExpresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eddh
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)
Plasmid#233876PurposeExpresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213DepositorInsertDadh
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH61 pLIB-8His3C-spt4
Plasmid#231649Purposeexpress S.cerevisiae spt4 in insect cellsDepositorInsertspt4 (SPT4 Budding Yeast)
UseTags8His-HRV3CExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1
Plasmid#228508PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I5CHO (#1)
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZB578_pB1H1_T7-RNAP_LARP-I
Plasmid#228507PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-tractin
Plasmid#187649PurposeExpresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.DepositorInsertF-tractin
UseAAVTagsmNeonGreenExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgSeLEU2
Plasmid#224870PurposeDisruption of S. eubayanus type LEU2 genesDepositorInsertpYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTrGCsgScLEU2
Plasmid#224869PurposeDisruption of S. cerevisiae type LEU2 genesDepositorInsertpYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgScLEU2
Plasmid#224868PurposeDisruption of S. cerevisiae type LEU2 geneDepositorInsertpYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDLterm
Plasmid#211903PurposeBase plasmid for dual-luciferase constructs to measure terminator strengthDepositorTypeEmpty backboneUseLuciferaseTagsExpressionPlantMutationPromoter35S enhancer + 35S minimal promoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
A99-ELP
Plasmid#170760PurposeA99 laminin α1 peptide fused to I30-ELP (VPGIG)30DepositorInsertA99-ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only