We narrowed to 3,066 results for: AER

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pJMP8930

Plasmid

#220905

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ptrc99a-pVhb-tdTER-fdh

Plasmid

#215649

Purpose

pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

trans-enoyl-CoA reductase
formate dehydrogenase

Expression

Bacterial

Promoter

Vhb

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pXWJ109Hg-gfp

Plasmid

#123148

Purpose

A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D

Plasmid

#51599

Purpose

expresses mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc tag

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
PXWABS62(E11-TEV)

Plasmid

#123157

Purpose

For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

ParsR-ABS62, Pecf11

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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As4

Plasmid

#123161

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J115-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J115, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEINO3

Plasmid

#179487

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

TetR/A

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQA-LP574

Plasmid

#222953

Purpose

Expresses R120A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 120 to Alanine

Promoter

T7

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPdelta

Plasmid

#13394

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPdelta (Cebpd Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-HRAS

Plasmid

#80604

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

HIV-1 p55 GAG

Expression

Mammalian

Promoter

SRalpha

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad1

Plasmid

#11735

Depositor

Jeff Wrana

Article

Hoodless et al Cell. 1996 May 17. 85(4):489-500.

Insert

SMAD1 (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-CFP

Plasmid

#22221

Purpose

Lentiviral expression of Arch-CFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

PQS1

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBTRCk-pVhb-maACR

Plasmid

#215651

Purpose

pBBR1 Origin, KanR, with maACR gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

acyl-CoA reductase

Tags

Maltose binding protein and His tag

Expression

Bacterial

Promoter

Vhb

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-pVhb-vfFadBA

Plasmid

#215650

Purpose

pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)

Expression

Bacterial

Promoter

Vhb

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-4

Plasmid

#160704

Purpose

Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

optimised synthetic mCherry

Use

Synthetic Biology

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization Control

Plasmid

#186553

Purpose

Negative Control mediating endosomal localization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-EGFP

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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