We narrowed to 2,435 results for: GCG

1. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-CENPA

   Plasmid

   #227282

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CENPA (CENPA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP gRNA (BRDN0000563266)

   Plasmid

   #80036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCRISPR-CMV-cGAS3

   Plasmid

   #102610

   Purpose

   CRISPR/Cas9 lentivector targeting human cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   gRNA targeting human cGAS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. FgH1tUTG_mup53_Ex5

   Plasmid

   #85535

   Purpose

   Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   mu p53
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG4K5

   Plasmid

   #74492

   Purpose

   For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. VCP F11.4 gRNA

    Plasmid

    #90938

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human VCP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    VCP (Guide Designation F11.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-Actb KI #2

    Plasmid

    #139666

    Purpose

    Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. shTBX5 # 2

    Plasmid

    #42550

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

13. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109316

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    eGFP gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2-LyzC-RacFRET-U6ac-ctrl guides

    Plasmid

    #168247

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv1 sgTollip

    Plasmid

    #196546

    Purpose

    Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPR v2-sgAMPK alpha1 clone1

    Plasmid

    #162119

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-sgApc/Cre

    Plasmid

    #89641

    Purpose

    Expresses an Apc-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Apc (Apc Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. E42_gRunx1_gRNA2_dTet_NGFR

    Plasmid

    #189801

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA2 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR-v1-sgHK2_2

    Plasmid

    #163461

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge