We narrowed to 24,476 results for: Spr;

1. pSL1211

   Plasmid

   #129521

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFlag-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMZ374

   Plasmid

   #59896

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBECKP-km

   Plasmid

   #117235

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCasKP-apr

   Plasmid

   #117231

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIMcpf1

   Plasmid

   #153034

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCasKP-hph

   Plasmid

   #117232

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCP-tRNA

   Plasmid

   #133812

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
   

   Use

   CRISPR

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYZ033

    Plasmid

    #98404

    Purpose

    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC0x_gRNA_Pos5_Ptet

    Plasmid

    #179336

    Purpose

    gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pG3H-U6SC

    Plasmid

    #134755

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-35SC

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Promoter

    S.pombe rrk1 and nmt41

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3K-U6SC

    Plasmid

    #134754

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA-U6, zCas9-35SC

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG3B-U3Ub

    Plasmid

    #134750

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC0x_gRNA_Pos4_Ptet

    Plasmid

    #179335

    Purpose

    gRNA Position Vector 4, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMC0x_gRNA_Pos5b2_Ptet

    Plasmid

    #179342

    Purpose

    gRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMC0x_gRNA_Pos5a1_Ptet

    Plasmid

    #179339

    Purpose

    gRNA Position Vector 5a1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pMC0x_gRNA_Pos5b1_Ptet

    Plasmid

    #179341

    Purpose

    gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pMC0x_gRNA_Pos5a2_Ptet

    Plasmid

    #179340

    Purpose

    gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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