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p415 Glo3 R59K ΔC

Plasmid

#112653

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔC

Expression

Yeast

Mutation

R59K, Δ431-493 (deletion of C-terminal amphipathi…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 A

Plasmid

#112656

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398A

Expression

Yeast

Mutation

R59K, mutations (S389A, S398A) in Glo3 motif, non…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 D

Plasmid

#112657

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398D

Expression

Yeast

Mutation

R59K, mutations (S389D, S398D) in Glo3 motif, pho…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 ΔGRM-ΔC

Plasmid

#112659

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 ΔGRM ΔC

Tags

sfGFP

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 A

Plasmid

#112660

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP- Glo3 S389,398A

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389A, S398A) in Glo3 motif, non-phosp…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 D

Plasmid

#112661

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 S389,398D

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389D, S398D) in Glo3 motif, phosphomi…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K L246D

Plasmid

#112648

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K L246D

Expression

Yeast

Mutation

R54K & L246D (in ALPS motif)

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K AxxA

Plasmid

#112649

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K AxxA

Expression

Yeast

Mutation

R54K & Alanine substitution of C-terminal δL …

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 Δ3xF AxxA

Plasmid

#112650

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 Δ3xF AxxA

Expression

Yeast

Mutation

R54K, W304A, W307A, W310A, W349A, F353A

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-cimA3.7

Plasmid

#134595

Purpose

Low Phosphate inducible mutant citramalate synthase

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

cimA3.7

Expression

Bacterial

Mutation

I47V, E114V, H126Q, T204A, L238S

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-SBS-mCherry

Plasmid

#134598

Purpose

Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

SBS-mCherry

Tags

mCherry

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-T7-CBD-hGLY

Plasmid

#134940

Purpose

T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Tags

chitin binding domain

Expression

Bacterial

Promoter

t7

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-β20cp6

Plasmid

#127060

Purpose

Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6-beta20

Tags

His tag

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

Plasmid

#124181

Purpose

piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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P415 δCLD+Ret2 2α

Plasmid

#73878

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD+Ret2 2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD+δC2α

Plasmid

#73877

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD+δC2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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miR373 in pcDNA3.1

Plasmid

#78127

Purpose

Mammalian expression of miR373

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR373 (MIR373 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Kif17 1-490 motor p201

Plasmid

#44706

Depositor

Geri Kreitzer

Article

Acharya et al J Biol Chem. 2013 Sep 26.

Insert

Kif17 (KIF17 Human)

Use

Gateway donor vector

Mutation

amino acids 1-490

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only