Addgene

We narrowed to 15,241 results for: grna

Showing: 1301 - 1320 of 15241 results

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ERN1 gRNA (BRDN0001162304)

Plasmid

#76407

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERN1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERN1 (ERN1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB4 gRNA (BRDN0001149495)

Plasmid

#77123

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB4 (PFKFB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK2A1 gRNA (BRDN0001146321)

Plasmid

#76675

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2A1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK2A1 (CSNK2A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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LIN52 D6.4 gRNA

Plasmid

#90735

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D6.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147805)

Plasmid

#77600

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA-POLQ-Halo

Plasmid

#211636

Purpose

sgRNA against POLQ

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA POLQ

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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CSNK2A1 gRNA (BRDN0001145419)

Plasmid

#76673

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2A1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK2A1 (CSNK2A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) TFRC

Plasmid

#74709

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CDK13 gRNA (BRDN0001147672)

Plasmid

#76625

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK13

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK13 (CDK13 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

C. elegans U6 snRNA pol III promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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MAP2K4 gRNA (BRDN0001145028)

Plasmid

#76650

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K4 (MAP2K4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Rad51AP1 B12.3 gRNA

Plasmid

#90869

Purpose

3rd generation lentiviral gRNA plasmid targeting human Rad51AP1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

Rad51AP1 (Guide Designation B12.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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ULK1 gRNA (BRDN0001145044)

Plasmid

#75736

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p(U6b-BsaI-gRNA)

Plasmid

#65956

Purpose

Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti‐puro‐OgRNA_mdxE53

Plasmid

#187054

Purpose

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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