We narrowed to 2,323 results for: hells

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HLH Bmal Pas 1-465-VenC L150E

Plasmid

#47366

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, L150E

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC L95E/L150E/W427A

Plasmid

#47372

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, L95E/L150E/W427A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC F423R

Plasmid

#47368

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, F423R

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC W427A

Plasmid

#47369

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, W427A

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC V435R

Plasmid

#47370

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, V435R

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC F423R/V435R

Plasmid

#47371

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, F423R/V435R

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-EF1α-BHLHE40-IRES-ZsGreen1

Plasmid

#153061

Purpose

Lentiviral vector expressing human BHLHE40

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-2200

Plasmid

#31062

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-2200 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Article

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCG_IFITM3

Plasmid

#179957

Purpose

Expression vector for human IFITM3.

Depositor

Frank Kirchhoff, Konstantin Sparrer

Article

Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

Insert

IFITM3 (IFITM3 Human)

Expression

Mammalian

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFastbac1-Flag-FANCD2

Plasmid

#134904

Purpose

codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

FANCD2 (FANCD2 Human)

Tags

Flag

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

polyhedron

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA (pLP140)

Plasmid

#168461

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

Depositor

Jef Boeke, Matthew Maurano

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mScarlet-Giantin

Plasmid

#196872

Purpose

Expresion of Giantin-fused to mScarlet. Used as Golgi marker

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mScarlet-Giantin (GOLGB1 Synthetic, Human)

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO-GOLGA3

Plasmid

#226451

Purpose

For subcloning of human GOLGA3 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

GOLGA3 (GOLGA3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Duet-1-NONO(53-312)

Plasmid

#135442

Purpose

Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Non-POU domain-containing octamer-binding protein (NONO Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pC1GFP-Kir7.1

Plasmid

#79118

Purpose

Express N-terminal fused Kir7.1 protein in mammalian cells

Depositor

Bikash Pattnaik

Article

Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi:

Insert

Inwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO-EP400

Plasmid

#226449

Purpose

For subcloning of human EP400 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EP400 (EP400 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFastbac-Dual-StrepII-BARD1

Plasmid

#137166

Purpose

Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

BARD1 (BARD1 Human)

Tags

StrepII

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

p10

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_A

Plasmid

#74376

Purpose

gRNA_A to knockout human AMPK alpha 2 using Cas9n.

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-WT

Plasmid

#74379

Purpose

Lentiviral expression vector containing flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Promoter

hSyn

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-ChR2-YFP

Plasmid

#178721

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP-PIGA2 (pLP300)

Plasmid

#168462

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

Depositor

Matthew Maurano

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HNF4A_P2-257

Plasmid

#31063

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-257 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge