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pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAB043c

Plasmid

#79223

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-p38-alpha; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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PI(DM)-Rac1(WT)dc1.FLARE

Plasmid

#87589

Purpose

PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Tags

MBP

Expression

Bacterial

Mutation

K196R

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pBS Alpha 2 globin

Plasmid

#19231

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 globin (HBA1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Expression

Bacterial

Promoter

Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9

Plasmid

#241827

Purpose

Expresses MMLVgag–dCas9 for producing RENDER-dCas9

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9

Use

CRISPR

Tags

FLAG, HA and TagBFP

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_Fsx1-V5_GFPnes

Plasmid

#240503

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

Fsx1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_EFF-1-V5_GFPnes

Plasmid

#240504

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

EFF-1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1

Plasmid

#236262

Purpose

Bacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

inserted GGT codon after starting ATG

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 R175E

Plasmid

#236263

Purpose

Bacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

R175E, inserted GGT codon after starting ATG

Promoter

lac

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Gag

Plasmid

#221325

Purpose

Dox-inducible expression of Gag in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Gag

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2_pJEx

Plasmid

#213908

Purpose

Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPI159

Plasmid

#207430

Purpose

Extrachromosomal expression for mNeonGreen Fusions N-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pPI143

Plasmid

#207427

Purpose

Extrachromosomal expression for mNeonGreen Fusions C-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

C-terminal mNeonGreen

Use

Unspecified

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-L676EG683E-del460-465_U

Plasmid

#147673

Purpose

Insect Expression of DmPAN3-L676EG683E-del460-465

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-L676EG683E-del460-465 (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA_T

Plasmid

#147634

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA (TNRC6C Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN3-L676EG683E-del460-465_U

Plasmid

#147658

Purpose

Insect Expression of DmPAN3-L676EG683E-del460-465

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-L676EG683E-del460-465 (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609_R

Plasmid

#147410

Purpose

Insect Expression of HsTNRC6C_1207-1609

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609 (TNRC6C Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-N-term_F

Plasmid

#146300

Purpose

Mammalian Expression of HsTNRC6A-Nterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-Nterm (TNRC6A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-C-term_F

Plasmid

#146301

Purpose

Mammalian Expression of HsTNRC6Biso1-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso1-Cterm (TNRC6B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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