We narrowed to 4,185 results for: Gca

1. pGL4.23-5xE-box

   Plasmid

   #206841

   Purpose

   The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   5x E-box ((GCCACGTGCA)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426_Cas9_gRNA-YPRCd15c

   Plasmid

   #87404

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. tet_pLKO.1_puro_shNRF2 #1

   Plasmid

   #136584

   Purpose

   Expresses an inducible short hairpin targeting human NRF2 sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shNRF2v1 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. STK11 gRNA (BRDN0001146880)

   Plasmid

   #75912

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK11

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK11 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV313

   Plasmid

   #204956

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   hph (hygromycin resistance marker)
   sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus fumigatus U3 promoter, Aspergillus ni…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426_Cas9_gRNA-ARS416d

   Plasmid

   #87386

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS416d sequence TAGTGCACTTACCCCACGTT in yeast chromosome 4.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS416d
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Nkx2-1*/Neo

   Plasmid

   #31272

   Depositor

   Monte Winslow, Tyler Jacks

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

    Plasmid

    #192684

    Purpose

    Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAPM-D4 miR30-AGO2 ts2

    Plasmid

    #115854

    Purpose

    AGO2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO2 shRNA (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDE710

    Plasmid

    #103020

    Purpose

    expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIB2 gRNA (BRDN0001144754)

    Plasmid

    #75602

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZAP70 gRNA (BRDN0001487050)

    Plasmid

    #77956

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAP70

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKIF1C-2xFLAG

    Plasmid

    #130979

    Purpose

    Expression of KIF1C-2xFLAG in mammalian cells.

    Depositor

    Anne Straube

    Article

    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

    Insert

    KIF1C-2xFLAG (KIF1C Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    4 silent mutations that make this construct resis…

    Promoter

    CMV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P-HA-Suv4-20h1mut

    Plasmid

    #86690

    Purpose

    For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    mutated region NHDC (asparagine 273 to cysteine 2…

    Promoter

    T3

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PRKCI gRNA (BRDN0001148989)

    Plasmid

    #76401

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCI

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCI (PRKCI Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRIM24 gRNA (BRDN0001148301)

    Plasmid

    #77888

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PIP4K2B gRNA (BRDN0001147019)

    Plasmid

    #77903

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP4K2B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP4K2B (PIP4K2B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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