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pFUW-tetO-RUNX1

Plasmid

#139821

Purpose

doxycycline-inducible expression of Human RUNX1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Runx1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW2086

Plasmid

#154335

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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FRB-TagRFPT-CAAX

Plasmid

#87710

Purpose

FRB-tagged TagRFPT; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FRB-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC9-C169S

Plasmid

#231673

Purpose

Expresses human ZDHHC9-C169S protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC9 (ZDHHC9 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJW2172

Plasmid

#163096

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTD79

Plasmid

#172900

Purpose

Neuronal promoter rgef-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11b

Plasmid

#139831

Purpose

doxycycline-inducible expression of mouse Bcl11b in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Bcl11b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Tcf4

Plasmid

#139806

Purpose

doxycycline-inducible expression of mouse Tcf4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Tcf4 (Tcf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-IKZF1

Plasmid

#139807

Purpose

doxycycline-inducible expression of Human IKZF1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Ikzf1 (IKZF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW2072

Plasmid

#154334

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTD80

Plasmid

#172901

Purpose

Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_NOPLight-ctr

Plasmid

#195579

Purpose

Expresses the control sensor NOPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight-ctr

Expression

Mammalian

Mutation

D110A, D130A

Promoter

CMV

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSynapsin1_NOPLight1

Plasmid

#195580

Purpose

Expresses NOPLight1 in neurons

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight1

Use

AAV

Expression

Mammalian

Promoter

human Synapsin-1

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJH126

Plasmid

#162482

Purpose

SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-GFI1

Plasmid

#139813

Purpose

doxycycline-inducible expression of Human GFI1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

GFI1 (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P2L- Please see depositor comment below

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT7[mRNA]-5'UTR:mMyod1[NM_010866.2]: Hba-a1_3'UTR

Plasmid

#205026

Purpose

In vitro transcription vector for production of synthetic mRNA encoding for mouse Myod1

Depositor

Ori Bar-Nur

Article

Qabrati et al NPJ Regen Med. 2023 Aug 8;8(1):43. doi:

Insert

Myod1 (Myod1 Mouse)

Use

Synthetic Biology; In vitro transcription vector …

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTD74

Plasmid

#172898

Purpose

Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTD82

Plasmid

#172903

Purpose

Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTD75

Plasmid

#172899

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on LGI

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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