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YFP(1-158) in pcDNAI/Amp

Plasmid

#60888

Purpose

This vector attaches YFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(1-158)

Use

Tags

Expression

Mammalian

Mutation

Met was substituted for Gln-69.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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YFP(159-238) in pcDNAI/Amp

Plasmid

#60889

Purpose

This vector attaches YFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(159-238)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-ExRai-CKAR2

Plasmid

#236097

Purpose

Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

ExRai-CKAR2-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-CKAR2

Plasmid

#236098

Purpose

Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyn-ExRai-CKAR2

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph2

Plasmid

#231902

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph1

Plasmid

#231901

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph0

Plasmid

#231900

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5

Plasmid

#235642

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_Fbl_N73_eMagBF_V5

Plasmid

#235643

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

Fbl-N73- and eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_downstream_sgRNA

Plasmid

#235610

Purpose

Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus downstream sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_upstream_sgRNA

Plasmid

#235609

Purpose

Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus upstream sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5_NES

Plasmid

#235614

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_BirA

Plasmid

#235634

Purpose

Encodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experiments

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

BirA

Use

Tags

NES-V5

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N65_eMagBF_V5_NES

Plasmid

#235612

Purpose

Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-65

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N77_eMagBF_V5_NES

Plasmid

#235616

Purpose

Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-77

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N99_eMagBF_V5_NES

Plasmid

#235618

Purpose

Encodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-99

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Esyn-FlpX-TVA950-EYFP-WPRE

Plasmid

#234710

Purpose

pAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFP

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

TVA950-eYFP

Use

AAV; Has fdio cassette for flp-dependent expressi…

Tags

Expression

Mammalian

Mutation

Promoter

enhanced Synapsin

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Use

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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