We narrowed to 3,322 results for: UBC

1. pET28a-shortSUMO1

   Plasmid

   #228951

   Purpose

   express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-17

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-RANGAP1-CTD

   Plasmid

   #228947

   Purpose

   express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   RANGAP1-CTD (RANGAP1 Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   contains aa 418-587 only

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

   Plasmid

   #236253

   Purpose

   Dre dependent NaChBac-TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

   Insert

   NaChBac-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4TO_24xGCN4_PCC_24xPP7

   Plasmid

   #236094

   Purpose

   Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   24xGCN4-PCC-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4TO_24xGCN4_MUT_24xPP7

   Plasmid

   #236093

   Purpose

   Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   24xGCN4-MUT-24xPP7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGEN_GFP

   Plasmid

   #235611

   Purpose

   Encodes GFP. For use in transfections as a negative control without epitope tags.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGEN_NES_V5_GFP

   Plasmid

   #235632

   Purpose

   Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   GFP
   

   Use

   Tags

   NES-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

   Plasmid

   #225064

   Purpose

   Expression of HalM-modified HalA2 in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   HalA2
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 PM-Ras-LOCKR-PL Key

   Plasmid

   #216504

   Purpose

   Ras activity dependent proximity labeler, Key only, plasma membrane localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   PM-Ras-LOCKR-PL Key
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Golgi-Ras-LOCKR-PL Key

    Plasmid

    #216505

    Purpose

    Ras activity dependent proximity labeler, Key only, Golgi localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Golgi-Ras-LOCKR-PL Key
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 ER-Ras-LOCKR-PL Key

    Plasmid

    #216506

    Purpose

    Ras activity dependent proximity labeler, Key only, ER localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    ER-Ras-LOCKR-PL Key
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Mito-Ras-LOCKR-PL Key

    Plasmid

    #216507

    Purpose

    Ras activity dependent proximity labeler, Key only, mitochondria localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Mito-Ras-LOCKR-PL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRC-N-Venus-V150A-apCC-Di

    Plasmid

    #190706

    Purpose

    Encodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.

    Depositor

    Nigel Savery

    Article

    Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

    Insert

    N-Venus-V150A-apCC-Di
    

    Use

    Tags

    Expression

    Mutation

    V150A

    Promoter

    araBAD

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSub-Asp6-MBP-HRas

    Plasmid

    #185758

    Purpose

    Bacterial expression of Asp6-MBP-HRas

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    Asp6-MBP-HRas
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    6x Asp added to the N terminus of MBP

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

    Plasmid

    #170792

    Purpose

    This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6p1 AO7R

    Plasmid

    #137842

    Purpose

    GST tagged AO7 encoding aa 126-216 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC_C_MRM1

    Plasmid

    #111671

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    MRM1 (MRM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCR8-DJ1-E64D

    Plasmid

    #29356

    Depositor

    Mark Cookson

    Article

    Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Glu64Asp

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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