We narrowed to 3,514 results for: Braf;
-
-
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQFDBD-2x AD*-VP16*, α-cry:EGFP
Plasmid#180473PurposeGene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFPDepositorInsertQFDBD-2xQFAD*-VP16*
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2b
Plasmid#109517PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2bDepositorInsertEHD2b (ehd2 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterflk1Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-C
Plasmid#201398PurposeExpresses C terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FusXTBE-1397-N
Plasmid#201397PurposeExpresses N terminus half of DddAtox in mammalian cellsDepositorInsertCOX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-foxc1a
Plasmid#132971Purposefull-length of foxc1a without stop codon in pmE Gateway vectorDepositorInsertfoxc1a (foxc1a Zebrafish)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p9xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180479PurposeInducible gene expression vector p9xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert9x QUAS-ZsGreen-P2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
p17xQUAS-Luciferase, α-cry:mCherry
Plasmid#180482PurposeInducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherryDepositorInsertsLuciferase
17x QUAS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorInsertEEA1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMini-CAAGs::RFP-DR48
Plasmid#132640PurposeFluorescent reporter to quantify elicitation of the strand annealing mediated repair pathwayDepositorInsertmRFP
UseTagsExpressionMammalianMutationContains a 92bp insertion interrupting the open …PromoterChicken B-actinAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1-DN
Plasmid#109536PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF1DepositorInsertARF1 (arf1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF6
Plasmid#109537PurposeMuscle specific zebrafish expression of mKate2 fused to ARF6DepositorInsertARF6 (arf6a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF6-DN
Plasmid#109538PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF6DepositorInsertARF6 (arf6a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
rag2:METTL121B_CE_pISceI
Plasmid#223030PurposeExpress METTL121B gene in mesenchymal lineage of Zebrafish.DepositorInsertMETTL121B
UseExpress the insert(gene) in mesenchymal lineage o…TagsExpressionMutationPromoterRag2Available sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
rag2:CTDSP2_CE_pISceI
Plasmid#223033PurposeExpress CTDSP2 gene in mesenchymal lineage of Zebrafish.DepositorInsertCTDSP2
UseExpress the insert(gene) in mesenchymal lineage o…TagsExpressionMutationPromoterRag2Available sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-A
Plasmid#221713PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD683A in active sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationY680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-E
Plasmid#221714PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME map2k2a_s219D
Plasmid#201058PurposeGateway middle entry vector expressing constitutively active zebrafish map2k2aDepositorInsertMAP2k2a (map2k2a Zebrafish)
UseZebrafish expressionTagsExpressionMutationMutation S219D, constitutively activePromoternoneAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TSAA
Plasmid#206179Purposein vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS192A, S194APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TAAS
Plasmid#206180Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S192APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TASA
Plasmid#206181Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194ADepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S194APromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1b
Plasmid#173126PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1b (kcnj1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj8
Plasmid#173134PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertUsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcryGFP-acta1-dmdGFP
Plasmid#172530PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.DepositorInsertdmd (dmd Zebrafish)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-jph1a
Plasmid#109497PurposeMuscle specific zebrafish expression of mKate2 fused to junctophilin 1aDepositorInsertjph1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1
Plasmid#109535PurposeMuscle specific zebrafish expression of mKate2 fused to ARF1DepositorInsertARF1 (arf1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Cav3-mKate2
Plasmid#109518PurposeMuscle specific zebrafish expression of mKate2 fused to Caveolin3DepositorInsertCav3 (cav3 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a-DN
Plasmid#109520PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2aDepositorInsertDnm2a (dnm2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-nsGFP
Plasmid#132975Purposegata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backboneDepositorInsertsnuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only
