We narrowed to 21,799 results for: Sis
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pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorInsertHSPH1 (HSPH1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
UseTagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialMutationPromotertacAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUB-sSypHer (RbcS-SypHer)
Plasmid#84732PurposeExpresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stomaDepositorInsertsSypHer
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMDC32B-AtMIR390a-NbSu-2
Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE331
Plasmid#153240PurposeM1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T38S38
Plasmid#49524PurposeAmpicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertconstitutive TetR38
UseTagsExpressionMutationPromoterPtb38Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P750
Plasmid#49525PurposeAmpicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertP750
UseTagsExpressionMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMB1-B-TelR
Plasmid#204980PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
UseTagsSuper ecliptic pHlorin A227DExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE342
Plasmid#153251PurposeM8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BD-cop1at(335-675)
Plasmid#44973DepositorInsertBD-COP1 (COP1 Mustard Weed)
UseTagsGAL4 DNA-binding domainExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMVAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
RS17060_pMQ30_KO_Kan
Plasmid#185396PurposeNon-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11DepositorInsertsRR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS17055_pMQ30_KO_Kan
Plasmid#185398PurposeNon-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11DepositorInsertsRR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
UseTagsTwinStrep-3CExpressionInsectMutationPromoterpolyhedrinAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero9
Plasmid#187927PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero9DepositorInsertHero9
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D1-TelR
Plasmid#204983PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXIN-Ap1g1-FKBP
Plasmid#46946PurposeRetroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseRetroviralTagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianMutationPromoterAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.0
Plasmid#139477PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTagsExpressionMammalianMutationV413LPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)-8myc
Plasmid#99516PurposeTruncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags8xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xEGFP-mMeikin (E156R, R159E)
Plasmid#174710PurposeMammalian expression of mouse Meikin with Separase-resistant mutationsDepositorInsertMeikin (Meikin Mouse)
UseTags3xEGFPExpressionMammalianMutationE156R, R159EPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-35S
Plasmid#99861PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-CTR1M154A
Plasmid#53158PurposeExpresses human CTR1 M154A from blasticidin resistance retroviral vectorDepositorInsertCTR1 (SLC31A1 Human)
UseRetroviralTagsExpressionMammalianMutationM154APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE497
Plasmid#153249PurposeM6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(FLLFLNL)
Plasmid#40021DepositorInsertsT (ATG)
GFP
beta-globin intron
Neo
UseTagsExpressionMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
UseTagsExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(31-114)-9myc
Plasmid#99517PurposeTruncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(31-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA17_no stop
Plasmid#83855PurposeGateway donor vector for IAA17 with no stop codonDepositorInsertIAA17 (AXR3 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCDB179-LCC-ICCG-I6M
Plasmid#218690PurposeEncodes codon optimized LCC-ICCG I6M enzyme for bacterial expressionDepositorInsertLCC-ICCG
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-pol2-Smarcd1-Myc
Plasmid#227716PurposeExpresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.DepositorInsertSmarcd1 (Smarcd1 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterpol2Available sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1-L92F-Q94Y
Plasmid#218705PurposeEncodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expressionDepositorInsertPES-H1
UseTags10xHisExpressionBacterialMutationL92F-Q94YPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA332
Plasmid#214744PurposeLow copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC-ICCG-DAQI
Plasmid#218689PurposeEncodes codon optimized LCC-ICCG DAQI enzyme for bacterial expressionDepositorInsertLCC-ICCG-DAQI
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TfCut2-L32E-S113E-T237Q
Plasmid#218696PurposeEncodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expressionDepositorInsertTfCut2
UseTags10xHisExpressionBacterialMutationL32E-S113E-T237QPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only
