We narrowed to 2,550 results for: CCS

1. pLX304 PCSK5 (T288P)-V5 blast

   Plasmid

   #83101

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pLX304 PCSK5 (A270T)-V5 blast

   Plasmid

   #83102

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Alanine 270 to Threonine

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pLX304 PCSK5 (H516P)-V5 blast

   Plasmid

   #83103

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Histidine 516 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pFL-SV40-TAK1 5'-UTR

   Plasmid

   #115356

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   TAK1 5'-UTR (MAP3K7 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-SPA mimic WT

   Plasmid

   #92350

   Purpose

   To mimic SPA RNA processing and expression

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

   Insert

   SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX302 PCSK5 (T288P)-V5 puro

   Plasmid

   #98709

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GIV-FLAG L249P (LP)

   Plasmid

   #86097

   Purpose

   Expression GIV in mammalian cell

   Depositor

   Pradipta Ghosh

   Article

   Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

   Insert

   GIV/Girdin (CCDC88A Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   L249P

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR (EGFP) shPnky-2

   Plasmid

   #79142

   Purpose

   Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

   Depositor

   Daniel Lim

   Article

   Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

   Insert

   shPnky-2 (Gm30731 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for the shRNA and CMV for EGFP

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIR-Report-Luc-KLF4-K2S

   Plasmid

   #62980

   Purpose

   Translational reporter for KLF4

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 partial sequence (KLF4 Human)
   

   Use

   Luciferase

   Tags

   firefly luciferase

   Expression

   Mutation

   Fragment 446-911 of KLF4

   Promoter

   CMV

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMIR-Report-Luc-KLF4-K3S

    Plasmid

    #62981

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 911-1406 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pMIR-Report-Luc-KLF4-K4S

    Plasmid

    #62982

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 1406-1781 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-Luc-KLF4-K6S

    Plasmid

    #62983

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 2086-2639 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_TTmiRc2_3xflag_CHEK2

    Plasmid

    #136526

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CHEK2 (CHEK2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. tet-pLKO.1_puro_shJUND #2

    Plasmid

    #136583

    Purpose

    Expresses an inducible short hairpin targeting human JUND sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shJUNDv2 (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOUPc-Rh159-GFP

    Plasmid

    #179280

    Purpose

    All in one "tet-on" lentivirus vector that expresses rhesus cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. Rh159 is an ER resident protein that binds NKG2D activating ligands.

    Depositor

    Jeremy Kamil

    Article

    Zhang et al mBio. 2019 Dec 10;10(6). pii: mBio.02110

    Insert

    Rh159 fused to eGFP
    

    Use

    Lentiviral; All-in-one "tet" on lentivi…

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Responsive Element 3G

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOUPc-Rh159-HA

    Plasmid

    #179344

    Purpose

    All-in-on "tet-on" 3G lentiviral vector plasmid encoding rhesus cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA tag fused to its cytoplasmic tail

    Depositor

    Jeremy Kamil

    Article

    Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00

    Insert

    Rh159
    

    Use

    Lentiviral; All in one tet-on lentiviral vector (…

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    tet responsive element 3G

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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