We narrowed to 15,731 results for: LEA

1. pHis-TEV-Nsp15-H234A

   Plasmid

   #219529

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   H234A (CAC to GCG)

   Available Since

   May 27, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

   Plasmid

   #228530

   Purpose

   Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Photinus pyralis, Human, Synthetic)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-MCP-dNS-SRRM1-V5-mCherry

   Plasmid

   #235095

   Purpose

   SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
   

   Expression

   Mammalian

   Mutation

   The SRRM1 entry clone from DNASU was modified usi…

   Promoter

   CAG

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

   Plasmid

   #235096

   Purpose

   SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
   

   Expression

   Mammalian

   Mutation

   The SRRM1 entry clone from DNASU was modified usi…

   Promoter

   CAG

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHis-TEV-Nsp15

   Plasmid

   #187656

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

   Insert

   Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TALEN Backbone

   Plasmid

   #31179

   Depositor

   Feng Zhang

   Insert

   TALE Nuclease Backbone
   

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pBAD33.1-DpnII

   Plasmid

   #127413

   Purpose

   Expresses DpnII restriction endonuclease in bacteria

   Depositor

   Douglas Feldman

   Insert

   DpnII
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOpen-T7EI

   Plasmid

   #165540

   Purpose

   DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   T7EI (T7 Endonuclease I)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pmCer-TopBP1

   Plasmid

   #211412

   Purpose

   mammalian expression of human TopBP1 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   TopBP1 (TOPBP1 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmCer-RecQL4

    Plasmid

    #211400

    Purpose

    mammalian expression of human RecQL4 N-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    RecQL4 (RECQL4 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET His6 prescission LIC cloning vector (HPKS)

    Plasmid

    #29720

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis and Prescission cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIC28-Bsa4-iLOVf

    Plasmid

    #63722

    Purpose

    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Tags

    TEE, iLOV, 6xHis, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 371-Bc

    Plasmid

    #97028

    Purpose

    yORF-tev-His6

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 371-B

    Plasmid

    #97027

    Purpose

    His6-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 371-R

    Plasmid

    #97033

    Purpose

    StrepII-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    StrepII tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 371-L

    Plasmid

    #97031

    Purpose

    FLAG-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    FLAG tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET His6 MBP prescission LIC cloning vector (HMPKS)

    Plasmid

    #29721

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, MBP, and Prescission cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RAB5AA-c003

    Plasmid

    #175475

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-N215.

    Depositor

    Opher Gileadi

    Insert

    RAB5A (RAB5A Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIP4_1JL1

    Plasmid

    #234206

    Purpose

    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1JL1 (rnhA Escherichia coli)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge