Addgene

We narrowed to 52,635 results for: Ran;

Showing: 1341 - 1360 of 52635 results

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SiT-EGFP

Plasmid

#166943

Purpose

mammalian expression of N' Sialyl Transferase tagged with EGFP

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

Insert

Sialyl Transferase N-terminus

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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1.6 kB VEGF-luc

Plasmid

#29667

Depositor

Patricia D'Amore

Article

Shima et al J Biol Chem. 1996 Feb 16. 271(7):3877-83

Insert

1.6 kB VEGF

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pFU-no toxin-PE

Plasmid

#24149

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

PDGF-receptor-transmembrane domain (PDGFRB Human, Aequorea victoria)

Use

Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2010

Availability

Academic Institutions and Nonprofits only
Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pBSU6-shScr/CMV-eGFP

Plasmid

#89912

Purpose

Express shRNA Scrambled control

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Scrambled

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon K49E

Plasmid

#11943

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

K49E

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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ECYFP

Plasmid

#111204

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2001 Feb 9;276(6):3805-10.

Insert

ECFP-Xpress tag-GFP10C fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pSG75-β2AR-T4L-sfGFP

Plasmid

#102461

Purpose

Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 104455

Insert

β2AR

Use

Tags

His tag and sfGFP

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCSHSP:Cre

Plasmid

#30524

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

Cre recombinase (cre bacteriophage P1)

Use

Cre/Lox; Heat shock inducible

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
AAA

Plasmid

#78487

Purpose

2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

CAN1 contains no stop codon

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Use

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pBSU6-shTom40.3/CMV-eGFP

Plasmid

#89797

Purpose

Expresses shRNA against TOM40.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-pvdF WT

Plasmid

#187974

Purpose

Expresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Kenjic et al Arch Biochem Biophys. 2019 Mar 30;664:40

Insert

pvdF WT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
PB-TAC-ERP2

Plasmid

#80478

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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