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We narrowed to 3,574 results for: golden gate

Showing: 1341 - 1360 of 3574 results

  1. C103m

    Plasmid
    #121007
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121007-plasmid-map-sequence-id-236162

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  5. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

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  6. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Use
    Tags
    HisTag
    Expression
    Mutation
    Promoter
    T7
    Available since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

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  7. pAIP4_6WEY

    Plasmid
    #234234
    Purpose
    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234234-plasmid-map-sequence-id-464198

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  8. pAIP4_3D1B

    Plasmid
    #234230
    Purpose
    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3D1B (tas3 Fission Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234230-plasmid-map-sequence-id-464195

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  9. pAIP4_2ESK

    Plasmid
    #234232
    Purpose
    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2ESK (UBE2D2 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234232-plasmid-map-sequence-id-464196

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  10. pAIP4_5F66

    Plasmid
    #234226
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5F66 (PPIA Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234226-plasmid-map-sequence-id-464191

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  11. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  12. pAIP4_5EIP

    Plasmid
    #234188
    Purpose
    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EIP (mmi1 Fission Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234188-plasmid-map-sequence-id-464153

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  13. pAIP4_5KUW

    Plasmid
    #234183
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5KUW (PPIA Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234183-plasmid-map-sequence-id-464148

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  14. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  15. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  16. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  17. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  18. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

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  19. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

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  20. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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Showing: 1341 - 1360 of 3574 results