We narrowed to 3,800 results for: golden gate

1. C41m

   Plasmid

   #120974

   Purpose

   MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C61m

   Plasmid

   #120977

   Purpose

   MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   RpaR from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. C62m

   Plasmid

   #120978

   Purpose

   MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   TraR(W) from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. P61m

   Plasmid

   #120953

   Purpose

   MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).

   Depositor

   Richard Murray

   Insert

   Tac promoter Adam Meyer
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. P16m

   Plasmid

   #120940

   Purpose

   MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ohAB carb pCinLacO m
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. P32m

   Plasmid

   #120943

   Purpose

   MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   PlacO (Tim Lu, Matt Bennett, etc)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. P35m

   Plasmid

   #120946

   Purpose

   MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).

   Depositor

   Richard Murray

   Insert

   pLbda-CasRQi
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

    Plasmid

    #195046

    Purpose

    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Inserts

    crtE
    crtI
    crtYB
    

    Expression

    Yeast

    Promoter

    pADH2, pGPD, and pXYL

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV_AA098

    Plasmid

    #245320

    Purpose

    Cas9 CRISPRko all-in-one positive control; targets CD47

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD47 (CD47 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT_S1-H1

    Plasmid

    #218392

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4_3KB5

    Plasmid

    #234225

    Purpose

    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KB5 (TRIM72 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBHt2G-RFP

    Plasmid

    #107162

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Type

    Empty backbone

    Use

    Fungal grobacterium transformation plasmid, teste…

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Poliovirus 1 PV1 isolate 09_05_19 3C protease

    Plasmid

    #228647

    Purpose

    Bacterial expression of the Polio 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Polio virus 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pHEE-R-hyg9si

    Plasmid

    #222832

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHEE-R-hyg5A9si

    Plasmid

    #222835

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHEE-R-bar9si

    Plasmid

    #222834

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHEE-R-bar5A9si

    Plasmid

    #222837

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHEE-R-kan5A9si

    Plasmid

    #222836

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge