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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2_mSTING_gRNA_1

Plasmid

#196625

Purpose

Knock-out STING in murine cells, gRNA 1.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 1 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
SrtA-PDGFR Tm

Plasmid

#121168

Purpose

pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

SrtA-PDGFR

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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p8.91

Plasmid

#187441

Purpose

Lentiviral packaging plasmid expressing gag and pol.

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Inserts

Use

Lentiviral

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-APOE-E4-T2A-Puro

Plasmid

#190813

Purpose

Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Mutation

E4

Promoter

Tet-O

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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