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pDEST27-GST-hMSN

Plasmid

#211823

Purpose

Express human MSN in mammalian cells

Depositor

Haian Fu

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL14-GFP

Plasmid

#67406

Purpose

Mammalian expression of hArl14 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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343 pTol2-fli1epDest

Plasmid

#73486

Purpose

Tol2 vector with chimeric endothelial enhancer/promoter upstream of attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL5A-GFP

Plasmid

#67399

Purpose

Mammalian expression of hArl5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-HOXA1

Plasmid

#31209

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pDEST40-CES2A-ΔC-S227A

Plasmid

#200556

Purpose

Mammalian expression of secreted mouse CES2A S227A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARFRP1-GFP

Plasmid

#67408

Purpose

Mammalian expression of hArfrp with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/v5-DEST hGlut9

Plasmid

#18088

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut9 (SLC2A9 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only
pDEST-APIC-LexAGAD

Plasmid

#112807

Purpose

gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS436-pCSDest2-AcrIIC2Nme

Plasmid

#85712

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC2Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF1-GFP

Plasmid

#67390

Purpose

Mammalian expression of hArf1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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JN607: pMVP/SB-DEST

Plasmid

#121860

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 HA-PNRC1

Plasmid

#123294

Purpose

Mammalian expression of PNRC1 N-HA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Luciferase; Gateway cloning vector

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL11-GFP

Plasmid

#67407

Purpose

Mammalian expression of hArl11 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 442 T to C; aa C148R

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only