Addgene

We narrowed to 9,573 results for: HAE

Showing: 1361 - 1380 of 9573 results

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pCASP-3G124-HilA

Plasmid

#153324

Purpose

Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

3G124 DARPin but no expression

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-TTM

Plasmid

#127794

Purpose

Expresses the TTM/CYTH domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 1-200)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-P17A,P18A

Plasmid

#104364

Purpose

Expresses human IFITM3-P17A,P18A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

mutated amino acids as follows: P17A,P18A

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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miR-451 Trap

Plasmid

#61487

Purpose

to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

miR-451 Trap

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

bidirectional phosphoglycerate kinase promoter (b…

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-hIFITM3-deltaY20

Plasmid

#58463

Purpose

Expresses human IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49,50,83A

Plasmid

#58420

Purpose

Expresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, and 83 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pVRa04_1617

Plasmid

#49658

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF04_1617

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf259

Plasmid

#12947

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf259

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf260

Plasmid

#12948

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf260

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf262

Plasmid

#12950

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf262

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf265

Plasmid

#12953

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf265

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf203

Plasmid

#12891

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf203

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf204

Plasmid

#12892

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf204

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf210

Plasmid

#12898

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf210

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf211

Plasmid

#12899

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf211

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf213

Plasmid

#12901

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf213

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf214

Plasmid

#12902

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf214

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf215

Plasmid

#12903

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf215

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only