19,771 results
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Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
Plasmid#89347Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequencesDepositorInsertJurkat TCRalpha/beta alleles and CD3e and CD3z
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsXRN1
Plasmid#66596Purposeexpresses lambdaN-HA-tagged XRN1 in mammalian cellsDepositorAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGagMBPHis
Plasmid#42176DepositorInsertGag protein sequence is from HIV-1 clone NL4-3
Tags6xHIS and MBPExpressionBacterialAvailable SinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Stat3-C Flag pRc/CMV
Plasmid#8722DepositorInsertStat3-C (Stat3 Mouse)
TagsFlagExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders…Available SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pComb8
Plasmid#63889PurposeFor phage display of antibody libraries by fusion to modified geneVIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneVIIIExpressionBacterialPromoterlacZAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only