165,567 results

1. pAAV-Ef1a-Con/Fon-mCherry

   Plasmid

   #137132

   Purpose

   Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiFCt-2in1-CC

   Plasmid

   #105114

   Purpose

   ratiometric BiFC analysis in plants (transient transformation)

   Depositor

   Christopher Grefen

   Article

   Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

   Type

   Empty backbone

   Tags

   cYFP-Myc/nYFP-HA

   Expression

   Plant

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRT

   Plasmid

   #120274

   Purpose

   TA-cloning vector of PCR-products

   Depositor

   Ken Motohashi

   Article

   Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

   Type

   Empty backbone

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a- gfp-2a-puro

   Plasmid

   #129443

   Purpose

   expression of GFP and Puro R

   Depositor

   Oskar Laur

   Insert

   gfp-puro
   

   Use

   Lentiviral

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100835

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCGBE1-SpRY (pBM1571)

   Plasmid

   #170105

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

   Promoter

   CMV

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lyso-GA

   Plasmid

   #209874

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PJEBAN3

   Plasmid

   #203682

   Purpose

   Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   Yellow Fluorescent Protein (YFP)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73022

    Purpose

    Luminescent and fluorescent dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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