165,567 results

1. c-Jun

   Plasmid

   #187902

   Purpose

   Expresses c-Jun in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   c-Jun (JUN Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRADZ1

   Plasmid

   #47698

   Purpose

   General cloning vector for use in E. coli or D. radiodurans with lacZ

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   repU

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLXEBR-pTF-cKit

   Plasmid

   #114293

   Purpose

   TET-inducible expression of c-Kit

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   cKit (Kit Mouse)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

   Plasmid

   #197510

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Mutation

   ABE8e mutations in TadA and nSpRY(D10A/A61R)

   Promoter

   Cbh

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Blast GFP

   Plasmid

   #12269

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   EGFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestFLAGHA_HuB

   Plasmid

   #65755

   Purpose

   expresses FLAGHA-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1C-YFP

   Plasmid

   #139646

   Purpose

   Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

   Depositor

   David Cobrinik

   Article

   Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

   Insert

   stuffer
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   See Depositor comments

   Promoter

   CMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159872

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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