162,982 results

1. pLVX-Puro-TDP-43-M337V

   Plasmid

   #133759

   Purpose

   Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed methionine 337 to valine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Puro_siKD

   Plasmid

   #86695

   Purpose

   Tetracycline inducible expression of shRNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 TO

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HO-Poly-KanMX4-HO

   Plasmid

   #51662

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRFP-Ub

   Plasmid

   #11935

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin (RBX1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGF1-4eCOL2A1

   Plasmid

   #97210

   Purpose

   Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   COL2A1 regulatory elements

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_118

   Plasmid

   #133459

   Purpose

   U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH3.3_Cre-MS2x12

   Plasmid

   #205559

   Purpose

   Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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