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Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-shRNA
Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterhPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1P-iCAG.copGFP
Plasmid#66577PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENsDepositorInsertcopGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T1
Plasmid#80495PurposeAAVS1 TALEN expression vector (Left)DepositorInsertAAVS1 TALEN (Left)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T2
Plasmid#80496PurposeAAVS1 TALEN expression vector (Right)DepositorInsertAAVS1 TALEN (Right)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-MCS
Plasmid#80488Purposedonor vector for AAVS1 targeting (puromycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgAAVS1
Plasmid#184403PurposepX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGATDepositorInsertAAVS1-specific sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-1
Plasmid#129726PurposeExpressing SpCas9 and sgAAVS1-1DepositorInsertSpCas9 and sgAAVS1-1
UseCRISPRTagsP2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGK-AAVS1ZFNR
Plasmid#60915Purposeencodes zinc finger nuclease specific to AAVS1 locus (right)DepositorInsertAAVS1-ZFNR
UseTagsExpressionMutationPromoterPGKAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PGK-AAVS1ZFNL
Plasmid#60916Purposeencodes zinc finger nuclease specific to AAVS1 locus (left)DepositorInsertAAVS1-ZFNL
UseTagsExpressionMutationPromoterPGKAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only