We narrowed to 505 results for: SPY

1. SpyCatcher002-oHex

   Plasmid

   #124670

   Purpose

   Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHex
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyTag003-mClover3

   Plasmid

   #133453

   Purpose

   Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mClover3
   

   Use

   Tags

   GSGSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher002-oPent

   Plasmid

   #124664

   Purpose

   Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oPent
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP783_SpyCatcher-DNMT3A-CO

   Plasmid

   #211776

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavudin-DNMT3A
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpyC-GFP

   Plasmid

   #220191

   Purpose

   sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG42 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG42

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEXP5-aHL_spytag_his

   Plasmid

   #107334

   Purpose

   Monomeric SpyTag-6×-His alpha-hemolysin

   Depositor

   George Church

   Article

   Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   alpha-hemolysin-SpyTag-6xHis
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpyCatcher002-oHept

   Plasmid

   #124671

   Purpose

   Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHept
   

   Use

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP780_SpyCatcher-KRAB-CO

    Plasmid

    #211773

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-KRAB
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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