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L2109-FRB-GPA-20-11S

Plasmid

#222880

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc11S

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmEDC3_331-680_D

Plasmid

#146100

Purpose

Bacterial Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Insert

DmEDC3_331-680 (Edc3 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmTral_395-542_D

Plasmid

#146101

Purpose

Bacterial Expression of DmTral_395-542

Depositor

Elisa Izaurralde

Insert

DmTral_395-542 (tral Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-P(11)4

Plasmid

#185786

Purpose

Encodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ATA-P(11)4

Plasmid

#185787

Purpose

Encodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

ATA-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-P(11)4

Plasmid

#185789

Purpose

Encodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_1-694_B

Plasmid

#145951

Purpose

Bacterial Expression of DmBelle_1-694

Depositor

Elisa Izaurralde

Insert

DmBelle_1-694 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_299-694_B

Plasmid

#145952

Purpose

Bacterial Expression of DmBelle_299-694

Depositor

Elisa Izaurralde

Insert

DmBelle_299-694 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_299-798_B

Plasmid

#145953

Purpose

Bacterial Expression of DmBelle_299-798

Depositor

Elisa Izaurralde

Insert

DmBelle_299-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_695-798_B

Plasmid

#145954

Purpose

Bacterial Expression of DmBelle_695-798

Depositor

Elisa Izaurralde

Insert

DmBelle_695-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTd-4-15-13-11

Plasmid

#63836

Purpose

This plasmid is designed to validate the biophysical model of translational coupling

Depositor

Howard Salis

Article

Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

Insert

CFP, mRFP1 and GFPmut3b

Expression

Bacterial

Mutation

The intergenic distance between each gene is -4 n…

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pAS-11 (bulge 9-15)

Plasmid

#40846

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–bulge9-15

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAS-9 (bulge 9-11)

Plasmid

#40844

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–bulge 9-11

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

Plasmid

#117656

Purpose

Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Inserts

sfCherry2(1-10)
sfCherry2(11)-SpyCatcher002

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP-mouse-Caspase-11

Plasmid

#183358

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-11 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-BeYDV-LS-L-11

Plasmid

#197717

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV LSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-BeYDV-LRS-L-11

Plasmid

#197718

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV LRSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-WDV-LRS-L-11

Plasmid

#197720

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

WDV LRSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.6 (11-362)

Plasmid

#177847

Purpose

Bacterial Expression of SnRK2.6

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.6 (OST1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-WDV-LS-L-11

Plasmid

#197719

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

WDV LSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO AMPKa (11-281)

Plasmid

#177851

Purpose

Bacterial Expression of AMPKa1

Depositor

Karsten Melcher

Article

Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

Insert

AMPK a1 (PRKAA1 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SOX2 TALE Repressor (KRAB 11-75)

Plasmid

#42946

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

SOX2 TALE Repressor (KRAB 11-75)

Use

Lentiviral

Tags

2A-GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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